Abstract

In this work, chemical crosslinking with N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDC) was studied in type I collagen-chitosan (Col I-CS) membranes for their use in guided bone regeneration (GBR). The membranes were prepared in a wide range of Col I-CS proportions, produced in the form of films by the solvent evaporation technique and crosslinked with EDC. The membranes presented a rough surface that is adequate for cell adhesion and a compact cross-section to prevent some infiltration of unwanted cell tissues, according to scanning electron microscopy. The physicochemical characterization (thermogravimetric analysis, differential scanning calorimetry, uniaxial tensile test and in vitro degradation) showed that the crosslinked membranes are more resistant to dehydration, tensile strength and enzymatic digestion. The biological assays (adhesion, viability and morphology of cells) using human fetal osteoblasts (hFOB) indicated that the crosslinked membranes are not cytotoxic. Therefore, the chemical crosslinking with EDC improves the physicochemical properties of the membranes without affecting their biocompatibility, indicating that this type of membranes is a potential alternative to be used in GBR. In addition, this study also allowed to know the best type of crosslinked membranes, as a proposal for its application in GBR strategies.

中文翻译：

---

N-（3-二甲基氨基丙基）-N'-乙基碳二亚胺盐酸盐化学交联的I型胶原蛋白-壳聚糖膜的定向骨再生：对比研究

摘要

在这项工作中，在I型胶原壳聚糖（Col I-CS）膜中研究了N-（3-二甲基氨基丙基）-N'-乙基碳二亚胺盐酸盐（EDC）的化学交联，以用于引导性骨再生（GBR）。制备的膜具有多种Col I-CS比例，通过溶剂蒸发技术制成薄膜形式，并与EDC交联。根据扫描电子显微镜，膜呈现出足以使细胞粘附的粗糙表面和紧凑的横截面，以防止有害细胞组织的某些浸润。理化特性（热重分析，差示扫描量热法，单轴拉伸试验和体外）降解）表明交联的膜对脱水，拉伸强度和酶消化具有更强的抵抗力。使用人类胎儿成骨细胞（hFOB）的生物学测定（细胞粘附，活力和形态）表明，交联的膜无细胞毒性。因此，与EDC的化学交联可改善膜的物理化学性质，而不会影响其生物相容性，表明这种类型的膜是GBR的潜在替代品。此外，这项研究还使人们知道了最佳的交联膜类型，作为将其应用于GBR策略的建议。