Abstract

Lipid bodies are dynamic organelles of photosynthetic microalgae that can be used as the third generation resources for biofuel production. Biosynthesis of lipids can be influenced by different signalling processes. Visualisation of these processes can provide useful information about the fate and associated roles of lipid molecules in different biological systems. In photosynthetic organisms, however, studies of calcium ediated lipid biosynthesis is bottlenecked due to the limitation of proper and efficient technologies, which also include visualisation techniques. Currently, most studies to visualise lipid droplets in vivo have used traditional dyes, and proper visualisation of lipid drops is hindered by dye-specific limitations. This hurdle could be overcome by using recently developed aggregation-induced emission biooprobes. This review reveals current knowledge gaps in the studies of lipid drops and calcium ions in microalgae, as calcium signaling is important secondary messenger to detect a wide variety of environmental stimuli in plant and animal cells. To obtain insight into the mechanisms of these processes, the merits and demerits of currently available visualisation techniques for lipid drops and calcium are also detailed. Finally, opportunities and possibilities are proposed to recommend further improvement of techniques for detecting the role of calcium during lipid formation in microalgae for biofuel production.

中文翻译：

---

合成荧光探针可了解微藻类脂滴积累中的钙信号传导-最新评论

摘要

脂质体是光合微藻的动态细胞器，可用作生物燃料生产的第三代资源。脂质的生物合成可能受不同的信号传导过程影响。这些过程的可视化可以提供有关脂质分子在不同生物系统中的命运和相关作用的有用信息。然而，在光合生物中，由于适当和有效技术的局限性，其中还包括可视化技术，因此对钙化脂质生物合成的研究受到了瓶颈。目前，大多数研究使体内的脂质液滴可视化已经使用了传统染料，并且由于特定于染料的局限性阻碍了脂质滴的正确可视化。通过使用最近开发的聚集诱导的发射生物制剂可以克服这一障碍。这篇评论揭示了微藻中脂质滴和钙离子研究的当前知识空白，因为钙信号传导是检测动植物细胞中各种环境刺激的重要二级信使。为了深入了解这些过程的机制，还详细介绍了目前可用的脂滴和钙可视化技术的优缺点。最后，提出了机会和可能性，以建议进一步改进用于检测钙在微藻中的脂质形成过程中用于生物燃料生产的作用的技术。