PURPOSE The purpose of this study was to establish a mouse model of proximal fibular osteotomy (PFO), and to determine if PFO could delay degeneration of the medial compartment of the knee joint in a mouse model. METHODS An animal model of destabilization of the medial meniscus (DMM) was used to induce post-traumatic knee osteoarthritis (OA). PFO was performed to examine the effectiveness of PFO on protection against medial compartment knee OA. Micro-CT was used to observe osteosclerosis development in the subchondral bone, and Safranin O-fast green staining was used to evaluate the progression of articular cartilage destruction. The condylar-plateau angle (CPA) and anatomical femorotibial angle (aFTA) were measured to determine whether knee alignment was changed after PFO. RESULTS PFO treatment could decrease osteophyte formation and osteosclerosis development in the subchondral bone, as observed by micro-CT. The value of the ratio of trabecular bone volume to total volume (BV/TV) of DMM+PFO group was lower than that of DMM group. PFO also inhibited the progression of articular cartilage destruction. DMM + PFO group displayed decreased maximal and summed OA scores, as compared with DMM group. Moreover, the change of knee alignment was reduced by PFO, which might be the mechanism of PFO alleviating medial compartment knee OA. CONCLUSION Our results indicated that PFO could alleviate medial compartment knee OA in a mouse model.

中文翻译：

---

在小鼠模型中，腓骨近端截骨术可缓解内侧腔室膝骨关节炎。

目的本研究的目的是建立小鼠近端腓骨截骨术（PFO）的小鼠模型，并确定PFO是否可以延迟小鼠模型中膝关节内侧腔的退化。方法使用破坏内侧半月板（DMM）的动物模型来诱发创伤后膝骨关节炎（OA）。进行PFO来检查PFO预防内侧膝关节OA的有效性。Micro-CT用于观察软骨下骨的骨硬化发展，番红花O-fast绿色染色用于评估关节软骨破坏的进展。测量P突平台角（CPA）和解剖型股胫角（aFTA），以确定PFO后膝关节的排列是否发生改变。结果如显微CT所观察到的，PFO治疗可以减少软骨下骨中骨赘的形成和骨硬化的发展。DMM + PFO组的小梁骨体积与总体积之比（BV / TV）的值低于DMM组。PFO还抑制了关节软骨破坏的进程。与DMM组相比，DMM + PFO组显示的最大和总OA分数降低。而且，PFO减少了膝关节排列的改变，这可能是PFO缓解内侧隔膝OA的机制。结论我们的结果表明PFO可以减轻小鼠模型中的内侧隔膝OA。PFO还抑制了关节软骨破坏的进程。与DMM组相比，DMM + PFO组显示的最大和总OA分数降低。而且，PFO减少了膝关节排列的改变，这可能是PFO缓解内侧膝关节OA的机制。结论我们的结果表明PFO可以减轻小鼠模型中的内侧隔膝OA。PFO还抑制了关节软骨破坏的进程。与DMM组相比，DMM + PFO组显示的最大和总OA分数降低。而且，PFO减少了膝关节排列的改变，这可能是PFO缓解内侧隔膝OA的机制。结论我们的结果表明PFO可以减轻小鼠模型中的内侧隔膝OA。