One-step, rapid fluorescence sensing of fungal viability based on a bioprobe with aggregation-induced emission characteristics,Materials Chemistry Frontiers

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One-step, rapid fluorescence sensing of fungal viability based on a bioprobe with aggregation-induced emission characteristics
Materials Chemistry Frontiers ( IF 6.0 ) Pub Date : 2020/01/20 , DOI: 10.1039/c9qm00732f
Xiaoxue Ge _{1,

2,

3,

4,

5} , Meng Gao _{4,

5,

6,

7} , Bo Situ _{1,

2,

3,

4,

5} , Weiwei Feng _{1,

2,

3,

4,

5} , Bairong He _{1,

2,

3,

4,

5} , Xiaojing He _{1,

2,

3,

4,

5} , Shiwu Li _{4,

5,

7,

8,

9} , Zihao Ou _{1,

2,

3,

4,

5} , Yiqi Zhong _{1,

2,

3,

4,

5} , Yahui Lin _{1,

2,

3,

4,

5} , Xinyi Ye _{1,

2,

3,

4,

5} , Xiumei Hu _{1,

2,

3,

4,

5} , Ben Zhong Tang _{4,

5,

7,

8,

9} , Lei Zheng _{1,

2,

3,

4,

5}

Affiliation

Fungi play key roles in various fields, including fermentation-based industries, medicine, and public health. The rapid and precise evaluation of fungal viability is critical for fungi-related fields. However, the methods currently applied have a series of limitations, such as time-consuming, laborious, and cumbersome. In this study, we designed and synthesized a water-dispersible fluorescent probe DPASI with typical aggregation-induced emission (AIE) characteristics for selectively lighting up dead Candida cells within 5 minutes. The rapid staining of the dead Candida cells could be ascribed to the efficient binding of DPASI with mitochondria, which was revealed by high-resolution fluorescence images taken by a structured illumination microscope (SIM) and colocalization images taken by a confocal laser scanning microscope (CLSM). Compared with traditional probes for the identification of dead Candida cells, the DPASI probe does not require tedious centrifugation and washing steps and has significant advantages in time-and-labor-saving. It thus provides a facile and promising tool for the rapid evaluation of antifungal susceptibility and screening of new antifungal drugs, which would greatly contribute to the fungal research.

中文翻译：

基于具有聚集诱导发射特性的生物探针，一步一步快速检测真菌活力

真菌在发酵工业，医药和公共卫生等各个领域发挥着关键作用。快速准确地评估真菌的生存能力对于真菌相关领域至关重要。但是，当前使用的方法具有一系列限制，例如费时，费力且麻烦。在这项研究中，我们设计并合成了具有典型聚集诱导发射（AIE）特性的水分散性荧光探针DPASI，用于在5分钟内选择性点亮死的念珠菌细胞。死者念珠菌的快速染色细胞可能归因于DPASI与线粒体的有效结合，这通过结构化照明显微镜（SIM）拍摄的高分辨率荧光图像和共聚焦激光扫描显微镜（CLSM）拍摄的共定位图像得以揭示。与用于鉴定死念珠菌细胞的传统探针相比，DPASI探针不需要繁琐的离心和洗涤步骤，并且在省时省力方面具有明显优势。因此，它为快速评估抗真菌药性和筛选新的抗真菌药提供了方便而有希望的工具，这将大大有助于真菌的研究。

更新日期：2020-03-05

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