One-step, rapid fluorescence sensing of fungal viability based on a bioprobe with aggregation-induced emission characteristics

Xiaoxue Ge; Meng Gao; Bo Situ; Weiwei Feng; Bairong He; Xiaojing He; Shiwu Li; Zihao Ou; Yiqi Zhong; Yahui Lin; Xinyi Ye; Xiumei Hu; Ben Zhong Tang; Lei Zheng

doi:10.1039/C9QM00732F

One-step, rapid fluorescence sensing of fungal viability based on a bioprobe with aggregation-induced emission characteristics†

Xiaoxue Ge,

‡^ab Meng Gao,

‡^c Bo Situ,

‡^ab Weiwei Feng,^ab Bairong He,^ab Xiaojing He,^ab Shiwu Li,^d Zihao Ou,

^ab Yiqi Zhong,^ab Yahui Lin,^ab Xinyi Ye,^ab Xiumei Hu,^ab Ben Zhong Tang

*^def and Lei Zheng

*^ab

Author affiliations

* Corresponding authors

^a Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China
E-mail: nfyyzhenglei@smu.edu.cn

^b Guangdong Engineering and Technology Research Center for Rapid Diagnostic Biosensors, Nanfang Hospital, Southern Medical University, Guangzhou, China

^c National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou, China

^d Center for Aggregation-Induced Emission, State Key Laboratory of Luminescent Materials and Devices, South China University of Technology, Guangzhou, China

^e Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, The Hong Kong University of Science & Technology, Clear Water Bay, Kowloon, Hong Kong, China
E-mail: tangbenz@ust.hk

^f HKUST Shenzhen Research Institute, No. 9 Yuexing 1st RD, South Area, Hi-tech Park, Nanshan, Shenzhen, China

Abstract

Fungi play key roles in various fields, including fermentation-based industries, medicine, and public health. The rapid and precise evaluation of fungal viability is critical for fungi-related fields. However, the methods currently applied have a series of limitations, such as time-consuming, laborious, and cumbersome. In this study, we designed and synthesized a water-dispersible fluorescent probe DPASI with typical aggregation-induced emission (AIE) characteristics for selectively lighting up dead Candida cells within 5 minutes. The rapid staining of the dead Candida cells could be ascribed to the efficient binding of DPASI with mitochondria, which was revealed by high-resolution fluorescence images taken by a structured illumination microscope (SIM) and colocalization images taken by a confocal laser scanning microscope (CLSM). Compared with traditional probes for the identification of dead Candida cells, the DPASI probe does not require tedious centrifugation and washing steps and has significant advantages in time-and-labor-saving. It thus provides a facile and promising tool for the rapid evaluation of antifungal susceptibility and screening of new antifungal drugs, which would greatly contribute to the fungal research.

This article is part of the themed collection: Recent Progress on Aggregation-Induced Emission

Materials Chemistry Frontiers

One-step, rapid fluorescence sensing of fungal viability based on a bioprobe with aggregation-induced emission characteristics†

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One-step, rapid fluorescence sensing of fungal viability based on a bioprobe with aggregation-induced emission characteristics

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