The oleaginous yeast Lipomyces starkeyi is a potential cost-effective source for the production of microbial lipids. Fatty acid elongases have vital roles in the syntheses of long-chain fatty acids. In this study, two genes encoding fatty acid elongases of L. starkeyi, LsELO1, and LsELO2 were identified and characterized. Heterologous expression of these genes in Saccharomyces cerevisiae revealed that LsElo1 is involved in the production of saturated long-chain fatty acids with 24 carbon atoms (C24:0) and that LsElo2 is involved in the conversion of C16 fatty acids to C18 fatty acids. In addition, both LsElo1 and LsElo2 were able to elongate polyunsaturated fatty acids. LsElo1 elongated linoleic acid (C18:2) to eicosadienoic acid (C20:2), and LsElo2 elongated α-linolenic acid (C18:3) to eicosatrienoic acid (C20:3). Overexpression of LsElo2 in L. starkeyi caused a reduction in C16 fatty acids, such as palmitic and palmitoleic acids, and an accumulation of C18 fatty acids such as oleic and linoleic acids. Our findings have the potential to contribute to the remodeling of fatty acid composition and the production of polyunsaturated long-chain fatty acids in oleaginous yeasts.

中文翻译：

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鉴定和鉴定Starlipi中的两个脂肪酸延伸酶。

产油酵母Lipomyces starkeyi是生产微生物脂质的潜在成本有效来源。脂肪酸延长酶在长链脂肪酸的合成中起着至关重要的作用。在这项研究中，两个编码L.starkeyi的脂肪酸延伸酶，LsELO1和LsELO2的基因被鉴定和鉴定。这些基因在酿酒酵母中的异源表达表明LsElo1参与了具有24个碳原子（C24：0）的饱和长链脂肪酸的生产，而LsElo2参与了C16脂肪酸向C18脂肪酸的转化。此外，LsElo1和LsElo2都能够延长多不饱和脂肪酸。LsElo1将亚油酸（C18：2）延长至二十碳二烯酸（C20：2），而LsElo2将α-亚麻酸（C18：3）延长至二十碳三烯酸（C20：3）。LsElo2在L中的过表达 starkeyi导致棕榈酸和棕榈油酸等C16脂肪酸的减少，以及油酸和亚油酸等C18脂肪酸的积累。我们的发现可能有助于油质酵母中脂肪酸组成的重塑和多不饱和长链脂肪酸的产生。