Abstract

We designed a hydrogen peroxide biosensor. The electrochemical detection of H₂O₂ was performed based on immobilization of cobalt nanoparticles–ferritin (CoNPs–Fer) onto multiwalled carbon nanotubes (MWCNTS) ensnared into chitosan (CS) matrices. Electrochemical techniques such as differential pulse voltammetry (DPV) and cyclic voltammetry (CV) was used to check the property of biosensor. Energy-dispersive X-ray spectroscopy (EDXS) and field emission scanning electron microscopy (FESEM) techniques showed the prosperous immobilization of CoNPs–Fer on the modified GC electrode surface. The hydrogen peroxide-designed biosensor displayed a linear range from 0.2 to 14 nM (R² = 0.99), a detection limit of 1.29 nM (S/N = 3) and sensitivity of –0.1105 μA/nM.The apparent heterogeneous electron transfer rate constant (K_s) and the charge transfer coefficient (α) were gained 4.19 s^–1 and 0.49, respectively. This biosensor can detect hydrogen peroxide with high sensitivity, selectivity, and low detection limit.

中文翻译：

---

基于蛋白质的纳米生物传感器电化学测定过氧化氢

摘要

我们设计了一个过氧化氢生物传感器。H ₂ O ₂的电化学检测是基于将钴纳米颗粒铁蛋白（CoNPs-Fer）固定在包裹在壳聚糖（CS）基质中的多壁碳纳米管（MWCNTS）上进行的。电化学技术，如差分脉冲伏安法（DPV）和循环伏安法（CV）用于检查生物传感器的性能。能量色散X射线光谱（EDXS）和场发射扫描电子显微镜（FESEM）技术表明，将CoNPs-Fer牢固地固定在修饰的GC电极表面上。过氧化氢设计的生物传感器的线性范围为0.2到14 nM（R ²= 0.99），检出限为1.29 nM（S / N = 3），灵敏度为–0.1105μA/ nM。获得的表观异质电子传输速率常数（K _s）和电荷传输系数（α）为4.19 s ^{– 1}和0.49。这种生物传感器可以高灵敏度，高选择性和低检测限检测过氧化氢。