Novel single nucleotide polymorphism loci were identified for teak (Tectona grandis Linn. f.) using a combined Next Generation Sequencing approach on the Illumina MiSeq system with genotyping on the Agena MassARRAY iPLEX™ platform. Three hundred and forty four putative loci were identified through sequencing of DNA fragments following a modified double digest Restriction Associated DNA Sequencing method. One hundred and eighty nine loci successfully amplified in 172 individuals from 20 populations in Myanmar. A total of 33 loci were removed from the final panel as two showed evidence of deviation from Hardy–Weinberg equilibrium, 17 were significantly linked and 23 were non-polymorphic. For the remaining 156 loci, expected heterozygosity ranged from 0.041 to 0.456 and F_ST from 0.062 to 0.632. These genetic resources will prove useful for future studies into the population genetics and phylogeography of this important and iconic timber species.

在Illumina MiSeq系统上使用结合了新一代测序方法并在Agena MassARRAY iPLEX™平台上进行基因分型的柚木（Tectona grandis Linn。f。）鉴定了新的单核苷酸多态性基因座。通过使用改良的双消化限制性相关DNA测序方法对DNA片段进行测序，确定了344个推定基因座。在缅甸20个人群的172个个体中成功扩增了189个基因座。从最后一个面板中总共删除了33个基因座，因为两个证据表明偏离了Hardy-Weinberg平衡，有17个显着相关，而23个是非多态的。对于其余的156个基因座，预期的杂合度范围为0.041至0.456和F _ST从0.062到0.632。这些遗传资源将被证明对今后对该重要和标志性木材物种的种群遗传学和系统地理学进行研究很有用。