Abstract
Novel single nucleotide polymorphism loci were identified for teak (Tectona grandis Linn. f.) using a combined Next Generation Sequencing approach on the Illumina MiSeq system with genotyping on the Agena MassARRAY iPLEX™ platform. Three hundred and forty four putative loci were identified through sequencing of DNA fragments following a modified double digest Restriction Associated DNA Sequencing method. One hundred and eighty nine loci successfully amplified in 172 individuals from 20 populations in Myanmar. A total of 33 loci were removed from the final panel as two showed evidence of deviation from Hardy–Weinberg equilibrium, 17 were significantly linked and 23 were non-polymorphic. For the remaining 156 loci, expected heterozygosity ranged from 0.041 to 0.456 and FST from 0.062 to 0.632. These genetic resources will prove useful for future studies into the population genetics and phylogeography of this important and iconic timber species.
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This work was supported by the Australian Centre for International Agricultural Research (ACIAR), through a series of grants (FST/2014/028, FST/2015/007 and FST/2016/025).
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Dunker, B., Dormontt, E.E., van Dijk, Kj. et al. A set of 156 SNP markers for teak (Tectona grandis Linn. f.). Conservation Genet Resour 12, 205–207 (2020). https://doi.org/10.1007/s12686-019-01099-7
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DOI: https://doi.org/10.1007/s12686-019-01099-7