Purpose

The present study was aimed to develop a successful bioprocess through solid state fermentation (SSF) technique for the butyric acid production using agriculture wastes like wheat bran, rice polishings and molasses anaerobically by Clostridium tyrobutyricum DSM 2637.

Methods

Different physiochemical parameters like substrate: water ratio, inoculum volume, different ionic salts concentrations, an additive effect of molasses and corn steep liquor were optimized to boost the butyric acid production through SSF of the growth medium anaerobically by Clostridium tyrobutyricum DSM 2637 at 37 °C, pH 6.0. The fermentation of the growth medium was terminated after 72 h of incubation. Quantification of butyric acid was done by Deniges method.

Results

The growth medium containing rice polishings, molasses, and wheat bran when subjected to determine substrate: water ratio through SSF at 37 °C, pH 6.0 anaerobically by Clostridium tyrobutyricum DSM 2637 yielded 5.63, 5.5, 4.26 mg/100 g of butyric acid respectively.

Conclusions

The present investigation demonstrated that rice polishings supplemented with corn steep liquor is a good agricultural waste for butyric acid production. The SSF technique can efficiently and successfully be utilized for better yield of butyric acid on the pilot scale.

中文翻译：

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酪丁酸梭菌固态发酵将农业废弃物生物转化为丁酸

目的

本研究旨在通过固态发酵（SSF）技术开发成功的生物工艺，以利用农业废料（例如麦麸，大米上光剂和糖蜜）通过酪丁酸梭菌DSM 2637厌氧生产丁酸。

方法

优化了不同的理化参数，例如底物：水的比例，接种量，不同的离子盐浓度，糖蜜和玉米浆的添加效果，以通过酪氨酸梭菌DSM 2637在37°C下厌氧地通过生长培养基的SSF来提高丁酸的产量。，pH 6.0。温育72小时后终止生长培养基的发酵。丁酸的定量通过Deniges方法进行。

结果

当通过酪氨酸丁酸梭菌DSM 2637在37°C，pH 6.0厌氧条件下通过SSF确定底物：水的比例时，含有水稻上光剂，糖蜜和麦麸的生长培养基分别产生5.63、5.5、4.26 mg / 100 g丁酸。

结论

本研究表明，补充玉米浆的大米抛光剂是生产丁酸的良好农业废料。SSF技术可以有效且成功地用于中试规模，以提高丁酸的收率。