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Bioconversion of Agricultural Waste to Butyric Acid Through Solid State Fermentation by Clostridium tyrobutyricum

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Abstract

Purpose

The present study was aimed to develop a successful bioprocess through solid state fermentation (SSF) technique for the butyric acid production using agriculture wastes like wheat bran, rice polishings and molasses anaerobically by Clostridium tyrobutyricum DSM 2637.

Methods

Different physiochemical parameters like substrate: water ratio, inoculum volume, different ionic salts concentrations, an additive effect of molasses and corn steep liquor were optimized to boost the butyric acid production through SSF of the growth medium anaerobically by Clostridium tyrobutyricum DSM 2637 at 37 °C, pH 6.0. The fermentation of the growth medium was terminated after 72 h of incubation. Quantification of butyric acid was done by Deniges method.

Results

The growth medium containing rice polishings, molasses, and wheat bran when subjected to determine substrate: water ratio through SSF at 37 °C, pH 6.0 anaerobically by Clostridium tyrobutyricum DSM 2637 yielded 5.63, 5.5, 4.26 mg/100 g of butyric acid respectively.

Conclusions

The present investigation demonstrated that rice polishings supplemented with corn steep liquor is a good agricultural waste for butyric acid production. The SSF technique can efficiently and successfully be utilized for better yield of butyric acid on the pilot scale.

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References

  1. Hijova, E., Chmelarova, A.: Short chain fatty acids and colonic health. Bratisl. Lekarske Listy. 108, 354–358 (2007)

    Google Scholar 

  2. Zhang, C., Hua, E., Yang, E., Fangxiao, Y., Yujiu, M.: Current progress on butyric acid production by fermentation. Curr. Microbiol. 59, 656–663 (2009). https://doi.org/10.1007/s00284-009-9491-y

    Article  Google Scholar 

  3. Hara, H.: Physiological effects of short-chain fatty acid produced from prebiotics in the colon. Biosci. Microflora. 21, 35–42 (2002)

    Article  Google Scholar 

  4. Pouillart, P.R.: Role of butyric acid and its derivatives in the treatment of colorectal cancer and hemoglobinopathies. Life Sci. 63, 1739–1760 (1998)

    Article  Google Scholar 

  5. Jha, A.K., Li, J., Yuan, Y., Baral, N., Ai, B.: A review on bio-butyric acid production and its optimization. Int. J. agri. Biol. 16, 1019–1024 (2014) ISSN Online: 1814-9596 13-1259/2014/16-5-1019-1024

    Google Scholar 

  6. Dwidar, M., Park, J.Y., Mitchell, J.R., Sang, B.: The future of butyric acid in industry. Sci. World J. (2012) https://doi.org/10.1100/2012/471417

    Article  Google Scholar 

  7. Zigova, J., Sturdık, E.: Advances in biotechnological production of butyric acid. J. Ind. Microbiol. Biotechnol. 24, 153–160 (2000). https://doi.org/10.1038/sj.jim.2900795

    Article  Google Scholar 

  8. Solange, I., Mussatto, L.F., Ballesteros, S.M., José, A.T.: Use of agro-industrial wastes in solid-state fermentation processes, industrial waste. In: Prof. Kuan-Yeow Show (Ed.), pp. 121–140 (2012). ISBN: 978-953-51-0253-3

  9. Krishna, C.: Solid-state fermentation systems—an overview. Crit. Rev. Biotechnol. 25(1–2), 1–30 (2005). https://doi.org/10.1080/07388550590925383

    Article  Google Scholar 

  10. Khachatourians, G.G., Arora, D.K. (eds.): Elsevier Science, Netherlands (2001)

  11. Bhargav, S., Panda, B.P., Ali, M., Javed, S.: Solid-state fermentation: an overview. Chem. Biochem. Eng. 1, 49–70 (2008)

    Google Scholar 

  12. Chen, T., Zhang, L., Luo, G., Yuan, W.: Butyric acid production by Clostridium tyrobutyricum in sugar mixtures and corncob hydrolysate containing arabinose. Bioresource. 12, 7931–7942 (2017). https://doi.org/10.15376/biores.12.4.7931-7942

    Article  Google Scholar 

  13. Zhu, Y., Wu, Z., Yang, S.: Butyric acid production from acid hydrolysate of corn fibre by Clostridium tyrobutyricum in a fibrous-bed bioreactor. Process Biochem. 38, 657–666 (2002)

    Article  Google Scholar 

  14. Liu, S., Bischoff, K.M., Leathers, T.D., Qureshi, N., Rich, J.O., Hughes, S.R.: Butyric acid from anaerobic fermentation of lignocellulosic biomass hydrolysates by Clostridium tyrobutyricum strain RPT-4213. Bioresour. Technol. 143, 322–329 (2013)

    Article  Google Scholar 

  15. Jiang, L., Wang, J., Liang, S., Wang, X., Cen, P., Xu, Z.: Butyric acid fermentation in a fibrous bed bioreactor with immobilized Clostridium tyrobutyricum from cane molasses. Bioresour. Technol. 100, 3403–3409 (2009)

    Article  Google Scholar 

  16. Huang, J., Cai, J., Wang, J., Zhu, X., Huang, L., Yang, S., Xu, Z.: Efficient production of butyric acid from Jerusalem artichoke by immobilized Clostridium tyrobutyricum in a fibrous-bed bioreactor. Bioresour. Technol. 102, 3923–3926 (2011)

    Article  Google Scholar 

  17. Shafaghat, H., Najafpour, G.D., Rezaei, S.P., Sharifzadeh, M.: Optimal growth of Saccharomyces cerevisiae (PTCC 24860) on pretreated molasses for the ethanol production: the application of response surface methodology. J. Chem. Ind. Chem. Eng. Quart. 16, 199–206 (2010)

    Article  Google Scholar 

  18. Ambreen, N., Hanif, N.Q., Khatoon, S.: Chemical composition of rice polishing from different sources. Pak. Vet. J. 26, 190–192 (2006)

    Google Scholar 

  19. Sramkova, Z., Gregova, E., Strudik, E.: Chemical composition and nutritional quality of wheat grain. Acta Chim Slovaca. 2, 115–138 (2009)

    Google Scholar 

  20. Bergeres, J., Sivela, S.: Detection and enumeration of Clostridial spores related to cheese quality classical and new methods. Bull. Int. Dairy Fed. 251, 15–23 (1990)

    Google Scholar 

  21. Huang, Y.L., Wu, Z., Zhang, L., Cheuang, C.M., Yang, S.T.: Production of carboxylic acids from hydrolyzed corn meal by immobilized cell fermentation in a fibrous-bed bioreactor. Bioresour. Technol. 82, 51–59 (2002)

    Article  Google Scholar 

  22. Song, H., Eom, M.H., Lee, S., Lee, J., Cho, J.H., Seung, D.: Modeling of batch experimental kinetics and application to fed-batch fermentation of Clostridium tyrobutyricum for enhanced butyric acid production. Biochem. Eng. J. 53, 71–76 (2010). https://doi.org/10.1016/j.bej.2010.09.010

    Article  Google Scholar 

  23. Sjobloma, M., Leonidas, M., Paul, C., Ulrika, R.: Production of butyric acid by Clostridium tyrobutyricum (ATCC25755) using sweet sorghum stalks and beet molasses. Ind. Crops Products 74, 535–544 (2015)

    Article  Google Scholar 

  24. Martin, R., Igor, D., Radek, S., Michal, R.: Butyric acid production with Clostridium tyrobutyricum immobilised to PVA gel. Process. Biochem. 51, 704–708 (2016)

    Article  Google Scholar 

  25. Chao, M., Jianfa, O., Matthew, M., Sarah, M.f., Xiaoguang, M.L.: High production of butyric acid by Clostridium tyrobutyricum mutant. Front. Chem. Sci. Eng. 9, 369–375 (2015). https://doi.org/10.1007/s11705-015-1525-3

    Article  Google Scholar 

  26. Xiaoguang, L., Shang-Tian, Y.: Kinetics of butyric acid fermentation of glucose and xylose by Clostridium tyrobutyricum wild type and mutant. Process. Biochem. 41, 801–808 (2006)

    Article  Google Scholar 

  27. AOAC: Official Methods of Analysis, Association of Analytical Chemist. 15th edn, pp. 1121–1180. AOAC, Washington, DC (1990)

    Google Scholar 

  28. Deniges, G.: Detection and estimation of butyric acid. Ann. Chim. Anal. Appl. 23, 27–31 (1918)

    Google Scholar 

  29. Jiang, L., Wang, J., Liang, S.: Enhanced butyric acid tolerance and bioproduction by Clostridium tyrobutyricum immobilized in a fibrous bed bioreactor. Biotechnol. Bioeng. 108, 31–40 (2011)

    Article  Google Scholar 

  30. Dwidar, M., Kim, S., Jeon, B.S., Um, Y., Mitchell, R.J., Sang, B.I.: Co-culturing a novel Bacillus strain with Clostridium tyrobutyricum ATCC25755 to produce butyric acid from sucrose. Biotechnol. Biofuels. 6, 1–10 (2013). https://doi.org/10.1186/1754-6834-6-35

    Article  Google Scholar 

  31. Jiang, L., Wang, J., Liang, S., Wang, X., Cen, P., Xu, Z.: Production of butyric acid from glucose and xylose with immobilized cells of Clostridium tyrobutyricum in a fibrous-bed bioreactor. Appl. Biochem. Biotech. 160, 350–359 (2010). https://doi.org/10.1007/s12010-008-8305-1

    Article  Google Scholar 

  32. Winston Liggett, R., Koffler, H.: Corn steep liquor in microbiology. Bacteriol. Rev. 12, 297–311 (1948)

    Article  Google Scholar 

  33. Zhu, Y., Yang, S.T.: Adaptation of Clostridium tyrobutylicum for enhanced tolerance to butyric acid in a fibrous-bed bioreactor. Biotechnol. Prog. 19, 365–372 (2003)

    Article  Google Scholar 

Download references

Acknowledgements

We are thankful to Hi-Tech Feed Mills, Lahore for providing rice polishings and wheat bran free of cost. The study was funded by Dr. Abu saeed Hashmi (Grant No. 1).

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Authors and Affiliations

  1. Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore, Pakistan

    Tasleem Akhtar, Abu Saeed Hashmi, Muhammad Tayyab, Aftab Ahmed Anjum, Shagufta Saeed & Sakhawat Ali

  2. C/O Mehtab Ahmed, Village Aima Ladian P.O. Kala Gujran, Tehsil & Distt., Jhelum, Pakistan

    Tasleem Akhtar

  3. Department of Microbiology, University of Veterinary and Animal Sciences, Lahore, Pakistan

    Aftab Ahmed Anjum

  4. PCSIR Labs, Lahore, Pakistan

    Sakhawat Ali

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  1. Tasleem Akhtar
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  2. Abu Saeed Hashmi
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Correspondence to Tasleem Akhtar.

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Akhtar, T., Hashmi, A.S., Tayyab, M. et al. Bioconversion of Agricultural Waste to Butyric Acid Through Solid State Fermentation by Clostridium tyrobutyricum. Waste Biomass Valor 11, 2067–2073 (2020). https://doi.org/10.1007/s12649-018-0475-7

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  • DOI: https://doi.org/10.1007/s12649-018-0475-7

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