This paper describes the simple nanopatterning of proteins on polyelectrolyte surfaces using microcontact printing with a nanopatternable, hydrophilic composite nanomold. The composite nanomold was easily fabricated by blending two UV-curable materials composed of Norland Optical Adhesives (NOA) 63 and poly(ethylene glycol) dimethacrylate (PEG-DMA). NOA 63 provided stable nanostructure formation and PEG-DMA induced high wettability of proteins in the nanomold. Using the composite mold and functionalized surface with polyelectrolytes, the fluorescent, isothiocyanate-tagged, bovine serum albumin (FITC-BSA) was successfully patterned with 8 nm height and 500 nm width. To confirm the feasibility of the protein assay on a nanoscale, a glycoprotein-lectin assay was successfully demonstrated as a model system. As expected, the lectins correctly recognized the nano-patterned glycoproteins such as chicken ovalbumin. The simple preparation of composite nanomold and functionalized surface with a universal platform can be applied to various biomolecules such as DNA, proteins, carbohydrates, and other biomolecules on a nanoscale.

中文翻译：

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使用复合纳米模具和自组装的聚电解质多层蛋白质的纳米图案

本文介绍了使用可纳米图案化的亲水性复合纳米模进行微接触印刷，在聚电解质表面进行蛋白质的简单纳米图案化。通过将两种由Norland光学粘合剂（NOA）63和聚乙二醇二甲基丙烯酸酯（PEG-DMA）组成的紫外线固化材料掺混，可以轻松制造复合纳米模具。NOA 63提供了稳定的纳米结构形成，并且PEG-DMA诱导了纳米模具中蛋白质的高润湿性。使用复合模具和带有聚电解质的功能化表面，成功地将荧光，异硫氰酸酯标记的牛血清白蛋白（FITC-BSA）图案化为8 nm高和500 nm宽度。为了证实在纳米规模上进行蛋白质测定的可行性，糖蛋白-凝集素测定已成功地证明为模型系统。不出所料 凝集素正确识别了纳米图案的糖蛋白，例如鸡卵清蛋白。具有通用平台的复合纳米模具和功能化表面的简单制备可以应用于各种生物分子，例如DNA，蛋白质，碳水化合物和其他纳米规模的生物分子。