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Nanopatterning of proteins using composite nanomold and self-assembled polyelectrolyte multilayers

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Abstract

This paper describes the simple nanopatterning of proteins on polyelectrolyte surfaces using microcontact printing with a nanopatternable, hydrophilic composite nanomold. The composite nanomold was easily fabricated by blending two UV-curable materials composed of Norland Optical Adhesives (NOA) 63 and poly(ethylene glycol) dimethacrylate (PEG-DMA). NOA 63 provided stable nanostructure formation and PEG-DMA induced high wettability of proteins in the nanomold. Using the composite mold and functionalized surface with polyelectrolytes, the fluorescent, isothiocyanate-tagged, bovine serum albumin (FITC-BSA) was successfully patterned with 8 nm height and 500 nm width. To confirm the feasibility of the protein assay on a nanoscale, a glycoprotein-lectin assay was successfully demonstrated as a model system. As expected, the lectins correctly recognized the nano-patterned glycoproteins such as chicken ovalbumin. The simple preparation of composite nanomold and functionalized surface with a universal platform can be applied to various biomolecules such as DNA, proteins, carbohydrates, and other biomolecules on a nanoscale.

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Authors and Affiliations

  1. School of Chemical and Biological Engineering, Seoul National University, 151-742, Seoul, Korea

    Sung-Kyu Kim & Byung-Gee Kim

  2. Department of Chemical Engineering, Chungnam National University, 305-764, Daejeon, Korea

    Ji-Hye Lee & Chang-Soo Lee

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  1. Sung-Kyu Kim
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  2. Byung-Gee Kim
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  3. Ji-Hye Lee
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  4. Chang-Soo Lee
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Correspondence to Chang-Soo Lee.

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Kim, SK., Kim, BG., Lee, JH. et al. Nanopatterning of proteins using composite nanomold and self-assembled polyelectrolyte multilayers. Macromol. Res. 17, 232–239 (2009). https://doi.org/10.1007/BF03218685

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  • DOI: https://doi.org/10.1007/BF03218685

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