S.cerevisiae is an industrially important organism known for its ability to produce ethanol as the demand for ethanol is increasing day by day all over the world, the need to find better and alternative ways to increase ethanol production is also rising. In this work we have proposed such alternative but effective method for producing ethanol by S.cerevisiae. Here, we are reporting for the first time the effect of nitrosative stress on ethanol production. Under in vivo condition, nitrosative stress is marked by the modification of macromolecules in the presence of reactive nitrogen species (RNS). Our result showed that treated cells were more capable for ethanol production compared with untreated cells. Our result also showed enhanced alcohol dehydrogenase activity under stressed condition. Further ethanol production was also optimized by using Response Surface Methodology (RSM) with stressed cells. Further, production of ethanol with immobilized beads of stress affected Saccharomyces cerevisiae was also determined. Overall, the obtained data showed that under nitrosative stress, the maximum ethanol production is 34.4 g/l after 24 h and such higher production was observed even after several cycles of fermentation. This is the first report of this kind showing the relation between nitrosative stress and ethanol production in Saccharomyces cerevisiae which may have important industrial application.

中文翻译：

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亚硝化暴露的酿酒酵母对乙醇生产的优化

酿酒酵母（S.cerevisiae）是一种工业上重要的生物，以其生产乙醇的能力而闻名，因为全世界对乙醇的需求日益增长，寻找更好的替代方法来增加乙醇产量的需求也在增加。在这项工作中，我们提出了一种通过酿酒酵母生产乙醇的替代但有效的方法。。在这里，我们首次报道了亚硝化胁迫对乙醇生产的影响。在体内条件下，亚硝化应激的特征是在存在反应性氮素（RNS）的情况下大分子的修饰。我们的结果表明，与未处理的细胞相比，处理过的细胞更有能力产生乙醇。我们的结果还表明，在压力条件下，乙醇脱氢酶活性增强。还可以通过对应力细胞使用响应表面方法（RSM）来优化乙醇的进一步生产。此外，用固定化的应力珠生产乙醇会影响酿酒酵母（Saccharomyces cerevisiae）也被确定。总体而言，所获得的数据表明，在亚硝化胁迫下，24小时后最大乙醇产量为34.4 g / l，即使经过数个发酵周期也观察到了更高的产量。这是第一份此类报告，显示了酿酒酵母中亚硝化胁迫与乙醇生产之间的关系，这可能具有重要的工业应用。