Skip to main content
SpringerLink
Log in

Optimization of Ethanol Production using Nitrosative Stress Exposed S.cerevisiae

  • Original Paper
  • Published:
Cell Biochemistry and Biophysics Aims and scope Submit manuscript

Abstract

S.cerevisiae is an industrially important organism known for its ability to produce ethanol as the demand for ethanol is increasing day by day all over the world, the need to find better and alternative ways to increase ethanol production is also rising. In this work we have proposed such alternative but effective method for producing ethanol by S.cerevisiae. Here, we are reporting for the first time the effect of nitrosative stress on ethanol production. Under in vivo condition, nitrosative stress is marked by the modification of macromolecules in the presence of reactive nitrogen species (RNS). Our result showed that treated cells were more capable for ethanol production compared with untreated cells. Our result also showed enhanced alcohol dehydrogenase activity under stressed condition. Further ethanol production was also optimized by using Response Surface Methodology (RSM) with stressed cells. Further, production of ethanol with immobilized beads of stress affected Saccharomyces cerevisiae was also determined. Overall, the obtained data showed that under nitrosative stress, the maximum ethanol production is 34.4 g/l after 24 h and such higher production was observed even after several cycles of fermentation. This is the first report of this kind showing the relation between nitrosative stress and ethanol production in Saccharomyces cerevisiae which may have important industrial application.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

References

  1. Willaert, R. G. (2017). Yeast biotechnology. Fermentation, 3, 7–10.

    Article  Google Scholar 

  2. Mohd Azhar, S. H., Abdullah, R., & Jambo, S. A. et al. (2017). Yeasts in sustainable bioethanol production: a review. Biochemistry and Biophysics Reports, 10, 52–61.

    Article  Google Scholar 

  3. Verbelen, P. J., De Schutter, D. P., & Delvaux, F. et al. (2006). Immobilized yeast cell systems for continuous fermentation applications. Biotechnology Letters, 28, 1515–1525.

    Article  CAS  Google Scholar 

  4. Zaldivar, J., Nielson, J., & Olsson, L. (2001). Fuel ethanol production from lignocelluloses: a challenge for metabolic engineering and process integration. Applied Microbiology and Biotechnology, 56, 17–34.

    Article  CAS  Google Scholar 

  5. Nevoigt, E. (2008). Progress in metabolic engineering of Saccharomyces cerevisiae. Microbiology and Molecular Biology Reviews, 72, 379–412.

    Article  CAS  Google Scholar 

  6. Matallana, E., & Aranda, A. (2017). Biotechnological impact of stress response on wine yeast. Letters in Applied Microbiology, 64, 103–110.

    Article  CAS  Google Scholar 

  7. Pretorius, I. S. (2000). Tailoring wine yeast for the new millennium: novel approaches to the ancient art of winemaking. Yeast, 16, 675–729.

    Article  CAS  Google Scholar 

  8. Forman, H., Fukuto, J. M., & Miller, T. (2008). The chemistry of cell signaling by reactive oxygen and nitrogen species and 4-hydroxynonenal. Archives of Biochemistry and Biophysics, 477, 183–195.

    Article  CAS  Google Scholar 

  9. Palmer, R. M., Rees, D. D., & Ashton, D. S. (1980). L-arginine is the physiological precursor for the formation of nitric oxide in endothelium-dependent relaxation. Biochemical and Biophysical Research Communications, 153, 1251–1256.

    Article  Google Scholar 

  10. Kurutas, E. B. (2016). The importance of antioxidants which play the role in cellular response against oxidative/nitrosative stress: current state. Nutrition Journal, 15, 71 https://doi.org/10.1186/s12937-016-0186-5.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Bhattacharjee, A., Majumdar, U., & Maity, D. et al. (2010). Characterizing the effect of nitrosative stress in Saccharomyces cerevisiae. Archives of Biochemistry and Biophysics, 496, 109–116.

    Article  CAS  Google Scholar 

  12. Rogstam, A., Larsson, J. T., & Kjelgaard, P. (2007). Mechanisms of adaptation to nitrosative stress in Bacillus subtilis. Journal of Bacteriology, 189, 3063–3071.

    Article  CAS  Google Scholar 

  13. Fang, F. C. (2004). Antimicrobial reactive oxygen and nitrogen species: concepts and controversies. Nature Reviews Microbiology, 2, 820–832.

    Article  CAS  Google Scholar 

  14. Bhattacharjee, A., Majumdar, U., & Maity, D. et al. (2009). In vivo protein tyrosine nitration in S. cerevisiae: Identification of tyrosine-nitrated proteins in mitochondria. Biochemical and Biophysical Research Communications, 388, 612–617.

    Article  CAS  Google Scholar 

  15. Crow, J. P., Beckman, J. S., & McCord, J. M. (1995). Sensitivity of the essential zinc-thiolate moiety of yeast alcohol dehydrogenase to hypochlorite and peroxynitrite. Biochemistry, 34, 3544–3552.

    Article  CAS  Google Scholar 

  16. Claudia, L., Nataliya, L., & Müller, A. (2013). Redox proteomics uncovers peroxynitrite-sensitive proteins that help Escherichia coli to overcome nitrosative stress. The Journal of Biological Chemistry, 288, 19698–19714.

    Article  Google Scholar 

  17. Hagman, A., Sa¨ll, T., & Compagno, C., et al. (2013). Yeast ‘make-accumulate-consume’’ life strategy evolved as a mMulti-step process that predates the wholeg duplication. PLoS ONE, 8, e68734.

    Article  CAS  Google Scholar 

  18. Sayyad, S. F., Chaudhari, S. R., & Panda, B. P. (2015). Quantitative determination of ethanol in arishta by using UVvisible spectrophotometer. Pharmaceutical and Biological Evaluations, 2, 204–207.

    Google Scholar 

  19. Zakya, A. S., Pensupa, N., & Andrade-Eiroa, Á. (2017). A new HPLC method for simultaneously measuring chloride, sugars, organic acids and alcohols in food samples. Journal of Food Composition and Analysis, 56, 25–33.

    Article  Google Scholar 

  20. Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72, 248–254.

    Article  CAS  Google Scholar 

  21. Richard, L. T. (1998). The competitive inhibition of yeast alcohol dehydrogenase by 2,2,2-trifluoroethanol. Biochemical Education, 26, 239–242.

    Article  Google Scholar 

  22. Pereira, A. P., Estevinho, L. M., & Mendes-Faia, A. (2014). Mead production: fermentative performance of yeasts entrapped in different concentrations of alginate. Journal of the Institute Brewing, 120, 575–580.

    CAS  Google Scholar 

  23. Saha, S. P., & Ghosh, S. (2014). Optimization of xylanase production by Penicillium citrinum xym2 and application in saccharification of agro-residues. Biocatalysis and Agricultural Biotechnology, 3, 188–196.

    Article  Google Scholar 

  24. Dhawan, V. (2014). Reactive oxygen and nitrogen species: general considerations. In N. K. Ganguly, S. K. Jindal, S. Biswal, P. J. Barnes & R. Pawankar Eds., Studies on Respiratory Disorders (pp. 27–47). New York, NY: Humana Press.

    Chapter  Google Scholar 

  25. Staab, C. A., Alander, J., & Brandt, M. (2008). Reduction of S- nitroglutathione by alcohol dehydrogenase III is faciliated by substrate alcohols via direct cofactor recycling and leads to GSH- controlled formation of glutathione transferase inhibitors. Biochemical Journal, 413, 493–504.

    Article  CAS  Google Scholar 

  26. Gow, A. J., & Stamler, J. S. (1998). Reaction between nitric oxide and haemoglobin under physiological condition. Nature, 391, 169–173.

    Article  CAS  Google Scholar 

  27. Sahoo, R., Bhattacharjee, A., & Majumder, U. et al. (2009). A novel role of catalase in detoxificationo peroxinitrite in Saccharomyces cerevisiae. Biochemical and Biophysical Research Communications, 38, 5507–5511.

    Google Scholar 

  28. Liu, L., Hausladen, A., & Zeng, M., et al. (2001). A metabolic enzyme for S-nitrosothiol conserved from bacteria to humans. Nature, 410, 490–494.

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors acknowledge the University of North Bengal for providing essential infrastructure and financial support to carry out this research.

Author information

Authors and Affiliations

  1. Department of Microbiology, University of North Bengal, Siliguri, India

    Swarnab Sengupta, Minakshi Deb, Rohan Nath, Shyama Prasad Saha & Arindam Bhattacharjee

Authors
  1. Swarnab Sengupta
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Minakshi Deb
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Rohan Nath
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Shyama Prasad Saha
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Arindam Bhattacharjee
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Arindam Bhattacharjee.

Ethics declarations

Conflict of Interest

The authors declare that they have no conflict of interest.

Additional information

Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Sengupta, S., Deb, M., Nath, R. et al. Optimization of Ethanol Production using Nitrosative Stress Exposed S.cerevisiae. Cell Biochem Biophys 78, 101–110 (2020). https://doi.org/10.1007/s12013-019-00897-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12013-019-00897-y

Keywords

Search

Navigation