In this study, we demonstrate that the biological unnatural amino acid incorporation method can be utilizedin vivo to synthesize an alkyne-terminated telechelic protein. Synthesis of terminally-functionalized polymers such as telechelic polymers is recognized to be important, since they can be employed usefully in many areas of biology and material science, such as drug delivery, colloidal dispersion, surface modification, and formation of polymer network. The introduction of alkyne groups into polymeric material is particularly interesting since the alkyne group can be a linker to combine other materials using click chemistry. To synthesize the telechelic recombinant protein, we attempted to incorporate the L-homopropargylglycine into the recombinant GroES fragment by expressing the recombinant gene encoding Met at the codons for both N- and C-terminals of the protein in the Met auxotrophicE. coli via Hpg supplementation. The Hpg incorporation rate was investigated and the incorporation was confirmed by MALDI-TOF analysis of the telechelic recombinant protein.

中文翻译：

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炔烃封端的远螯重组蛋白的生物合成

在这项研究中，我们证明了生物非天然氨基酸掺入方法可以在体内利用合成炔烃封端的远螯蛋白。端基官能化聚合物（例如远螯聚合物）的合成被认为是重要的，因为它们可以有效地用于生物学和材料科学的许多领域，例如药物递送，胶体分散，表面改性和聚合物网络的形成。将炔基基团引入聚合材料中特别令人感兴趣，因为炔基基团可以是通过点击化学结合其他材料的连接基。为了合成远螯重组蛋白，我们尝试通过在大都会营养缺陷型大肠杆菌的蛋白质N和C末端的密码子处表达编码Met的重组基因，将L-高炔丙基甘氨酸掺入重组GroES片段中。通过Hpg补充。研究了Hpg的掺入率，并通过远螯重组蛋白的MALDI-TOF分析确认了掺入。