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Biological synthesis of alkyne-terminated telechelic recombinant protein

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Abstract

In this study, we demonstrate that the biological unnatural amino acid incorporation method can be utilizedin vivo to synthesize an alkyne-terminated telechelic protein. Synthesis of terminally-functionalized polymers such as telechelic polymers is recognized to be important, since they can be employed usefully in many areas of biology and material science, such as drug delivery, colloidal dispersion, surface modification, and formation of polymer network. The introduction of alkyne groups into polymeric material is particularly interesting since the alkyne group can be a linker to combine other materials using click chemistry. To synthesize the telechelic recombinant protein, we attempted to incorporate the L-homopropargylglycine into the recombinant GroES fragment by expressing the recombinant gene encoding Met at the codons for both N- and C-terminals of the protein in the Met auxotrophicE. coli via Hpg supplementation. The Hpg incorporation rate was investigated and the incorporation was confirmed by MALDI-TOF analysis of the telechelic recombinant protein.

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Authors and Affiliations

  1. Institute for Environmental Technology and Industry, Pusan National University, 609-735, Busan, Korea

    Niraikulam Ayyadurai, So-Yeon Kim & Sun-Gu Lee

  2. School of Biotechnology, Yeungnam University, 712-749, Gyeongsan, Korea

    Niraikulam Ayyadurai, So-Yeon Kim & Sun-Gu Lee

  3. Department of Polymer Science and Chemical Engineering, Pusan National University, 609-735, Busan, Korea

    Soundrarajan Nagasundarapandian, Aleya Hasneen & Hyun-Jong Paik

  4. Gachon BioNano Research Institute, Kyungwon University, 461-701, Gyeonggi-do, Korea

    Seong Soo A. An

  5. Graduate School of Global Entrepreneurship, Hoseo University, 336-795, Chungnam, Korea

    Eugene Oh

Authors
  1. Niraikulam Ayyadurai
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  2. So-Yeon Kim
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  3. Sun-Gu Lee
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  4. Soundrarajan Nagasundarapandian
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  5. Aleya Hasneen
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  6. Hyun-Jong Paik
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  7. Seong Soo A. An
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  8. Eugene Oh
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Correspondence to Sun-Gu Lee.

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These authors contributed equally to this work.

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Ayyadurai, N., Kim, SY., Lee, SG. et al. Biological synthesis of alkyne-terminated telechelic recombinant protein. Macromol. Res. 17, 424–429 (2009). https://doi.org/10.1007/BF03218884

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  • DOI: https://doi.org/10.1007/BF03218884

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