We developed two quantitative PCR assays for use with environmental DNA (eDNA) to detect numerous species in the genus Cottus that are indigenous to the Pacific coast watersheds of North America. We conducted in vitro assay validations on ten Cottus species and 32 potentially co-occurring non-Cottus species. We demonstrate the efficacy of these assays by field testing eDNA samples collected from streams inhabited by Cottus. These assays will be particularly useful for detecting Cottus in habitats where one (or more) of several Cottus species could be present.

中文翻译：

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开发了两种定量PCR分析方法，用于从北美太平洋沿岸流域的环境DNA中检测几种Cottus物种

我们开发了两种定量PCR分析法，可与环境DNA（eDNA）一起使用，以检测Cottus属中北美北太平洋沿岸流域的众多物种。我们对10种Cottus物种和32种可能同时存在的非Cottus物种进行了体外测定验证。我们通过现场测试从Cottus居住的河流收集的eDNA样品证明了这些测定的有效性。这些测定对于在可能存在几种（一种或多种）Cottus物种的栖息地中检测Cottus尤其有用。