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Development of two quantitative PCR assays for detection of several Cottus species from environmental DNA in Pacific coast watersheds of North America

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A Correction to this article was published on 21 November 2019

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Abstract

We developed two quantitative PCR assays for use with environmental DNA (eDNA) to detect numerous species in the genus Cottus that are indigenous to the Pacific coast watersheds of North America. We conducted in vitro assay validations on ten Cottus species and 32 potentially co-occurring non-Cottus species. We demonstrate the efficacy of these assays by field testing eDNA samples collected from streams inhabited by Cottus. These assays will be particularly useful for detecting Cottus in habitats where one (or more) of several Cottus species could be present.

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Change history

  • 21 November 2019

    In the original publication of the article, the latitude and longitude values of the eDNA sample site, “Thornton Creek, WA” were published incorrectly in Table 2. The corrected Table 2 is given below.

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Acknowledgements

Funding for this work was provided by Seattle City Light agreement #18WNTAAYD00SCL. Cottus samples were obtained from the University of Washington Burke Museum Fish Collection. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government.

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  1. Western Fisheries Research Center, USGS, Seattle, WA, USA

    Marshal S. Hoy & Carl O. Ostberg

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  1. Marshal S. Hoy
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  2. Carl O. Ostberg
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Correspondence to Marshal S. Hoy.

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Hoy, M.S., Ostberg, C.O. Development of two quantitative PCR assays for detection of several Cottus species from environmental DNA in Pacific coast watersheds of North America. Conservation Genet Resour 12, 361–363 (2020). https://doi.org/10.1007/s12686-019-01118-7

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  • DOI: https://doi.org/10.1007/s12686-019-01118-7

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