Sperm cryopreservation is a routine method in andrology and IVF laboratory. However, the sperm quality and its fertilizing capacity have been decreased during this process. The purpose of this experiment was to determine the role of myoinositol as a supplement in amelioration of total and progressive sperm motility, DNA fragmentation, total antioxidant capacity (TAC), reactive oxygen species (ROS), and lipid peroxidation after the freezing–thawing process on patients with oligoasthenoteratozoospermia (OAT) syndrome. Semen samples obtained from 40 patients were divided into two aliquots and freezed with simple and 2 mg/mL myoinositol (MYO) supplemented freezing media. All samples were thawed and assessed after one month. Semen parameters were analyzed in terms of the motility by CASA, the level of total ROS by fluorimetry, TAC and MDA by colorimetric assay and finally DNA fragmentation by TUNEL assay. Our results clearly showed that MYO could improve total (37.46 vs. 12.91, p < 0.001) and progressive motility (21.92 vs. 6.49, p < 0.001) in experimental group compared to control group. A higher TAC level was observed in the MYO treated group in comparison to control group (1.11 vs. 0.91, p = 0.05). While MYO supplementation could not be effective on ROS level, it reduced DNA fragmentation of sperm after freeze–thaw process (p = 0.01). Therefore, MYO could be a good supplement for sperm freezing to reduce the detrimental effects of freezing process especially on DNA integrity, which is an important factor in the success of ART, in OAT suffered patients.

中文翻译：

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冷冻解冻过程后，肌醇补充剂是否能改善少脂非小动物精子症患者的精子参数和DNA完整性？

精子冷冻保存是男科和体外受精实验室的常规方法。但是，在此过程中精子质量及其受精能力下降。本实验的目的是确定肌醇在改善冻融过程中总和进行性精子运动，DNA片段化，总抗氧化能力（TAC），活性氧（ROS）和脂质过氧化作用方面的作用患有少脂少肌症（OAT）综合征的患者。将40例患者的精液样品分成两等份，并用简单的和2 mg / mL肌醇（MYO）补充的冷冻培养基冷冻。一个月后将所有样品融化并评估。通过CASA分析精液参数的活力，荧光法测定总ROS含量，通过比色法分析TAC和MDA，最后通过TUNEL法分析DNA片段化。我们的结果清楚地表明，MYO可以提高总数（37.46比12.91， 与对照组相比，实验组的p  <0.001）和进行性运动（21.92 vs. 6.49，p <0.001）。与对照组相比，MYO治疗组观察到更高的TAC水平（1.11比0.91，p  = 0.05）。尽管补充MYO不能有效提高ROS水平，但可以减少冻融过程后精子的DNA片段化（p  = 0.01）。因此，MYO可以作为精子冷冻的良好补充剂，以减少冷冻过程，尤其是对DNA完整性的有害影响，这是OAT受累患者抗逆转录病毒治疗成功的重要因素。