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Does myoinositol supplement improve sperm parameters and DNA integrity in patients with oligoasthenoteratozoospermia after the freezing–thawing process?

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Abstract

Sperm cryopreservation is a routine method in andrology and IVF laboratory. However, the sperm quality and its fertilizing capacity have been decreased during this process. The purpose of this experiment was to determine the role of myoinositol as a supplement in amelioration of total and progressive sperm motility, DNA fragmentation, total antioxidant capacity (TAC), reactive oxygen species (ROS), and lipid peroxidation after the freezing–thawing process on patients with oligoasthenoteratozoospermia (OAT) syndrome. Semen samples obtained from 40 patients were divided into two aliquots and freezed with simple and 2 mg/mL myoinositol (MYO) supplemented freezing media. All samples were thawed and assessed after one month. Semen parameters were analyzed in terms of the motility by CASA, the level of total ROS by fluorimetry, TAC and MDA by colorimetric assay and finally DNA fragmentation by TUNEL assay. Our results clearly showed that MYO could improve total (37.46 vs. 12.91, p < 0.001) and progressive motility (21.92 vs. 6.49, p < 0.001) in experimental group compared to control group. A higher TAC level was observed in the MYO treated group in comparison to control group (1.11 vs. 0.91, p = 0.05). While MYO supplementation could not be effective on ROS level, it reduced DNA fragmentation of sperm after freeze–thaw process (p = 0.01). Therefore, MYO could be a good supplement for sperm freezing to reduce the detrimental effects of freezing process especially on DNA integrity, which is an important factor in the success of ART, in OAT suffered patients.

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Acknowledgments

The authors would like to thank the Shahid Akbarabadi Clinical Research Development Unit (ShACRDU), Iran University of Medical Sciences (IUMS), Tehran, Iran for their financial support (No. 32641).

Funding

This study was funded by Shahid Akbarabadi Clinical Research Development Unit (ShACRDU), Iran University of Medical Sciences (IUMS), Tehran, Iran (grant number 32641).

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Authors and Affiliations

  1. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Mona Abdolsamadi

  2. Department of Anatomical Sciences, Iran University of Medical Science, Tehran, Iran

    Fatemeh Mohammadi, Reza Sardar, Maryam Haghighi & Zahra Zandieh

  3. Department of Infertility, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran

    Maryam Shabani Nashtaei

  4. Department of Clinical Biochemistry, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Maryam Teimouri

  5. Radiation Oncology Department, Emam Reza Hospital, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Maliheh Dayani

  6. Shahid Akbarabadi Clinical Research Development Unit (ShACRDU), Iran University of Medical Science, Tehran, Iran

    Samaneh Ghasemi & Zahra Zandieh

  7. Department of Comparative Biosciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

    Akram Vatannejad

  8. Student’s Scientific Research Center, Tehran University of Medical Sciences, Tehran, Iran

    Akram Vatannejad

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  1. Mona Abdolsamadi
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  2. Fatemeh Mohammadi
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  3. Maryam Shabani Nashtaei
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Correspondence to Akram Vatannejad or Zahra Zandieh.

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The study was approved by the Ethical Committee of Iran University of Medical Sciences (IR.IUMS.REC.1396.32641).

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Abdolsamadi, M., Mohammadi, F., Nashtaei, M.S. et al. Does myoinositol supplement improve sperm parameters and DNA integrity in patients with oligoasthenoteratozoospermia after the freezing–thawing process?. Cell Tissue Bank 21, 99–106 (2020). https://doi.org/10.1007/s10561-019-09801-7

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