The novel bacterial strains EF2 and OW1-1 were isolated from intestine of Eisenia fetida and municipal organic waste respectively. The qualitative screening of strains in carboxymethyl cellulose (CMC) agar plate showed a larger zone of clearance with Gram’s iodine staining. The 16S rRNA gene sequences of the strains EF2 and OW1-1 were identified as a gram-positive Bacillus sp. The strains exhibited significantly high CMCase activities of 35.307 ± 0.08 IU/ml and 29.92 ± 0.01 IU/ml, respectively, in EF2 and OW1-1 when 2.5% (w/v) of lactose was used as a carbon source at their optimal pH and temperature. The SDS-PAGE and zymogram analysis of crude enzyme revealed that the molecular weight of CMCase was 60 kDa in both strains. In comparing to monoculture of EF2 and OW1-1, the co-culture increased their activity by 15% and 35.71% respectively. The higher CMCase activity of strains in a wider range of temperatures and pH fluctuation could be a good choice for biofuel industries.

中文翻译：

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从城市污泥和红色摇摆虫蠕虫肠道分离的新型芽孢杆菌种共培养以提高CMCase活性

从fetida的小肠和城市有机废物中分别分离出新的菌株EF2和OW1-1 。对羧甲基纤维素（CMC）琼脂平板中的菌株进行定性筛选，结果显示革兰氏碘染色可清除更大的清除区域。菌株EF2和OW1-1的16S rRNA基因序列被鉴定为革兰氏阳性芽孢杆菌sp。当在最佳pH下使用2.5％（w / v）乳糖作为碳源时，菌株在EF2和OW1-1中分别显示出显着高的CMCase活性，分别为35.307±0.08 IU / ml和29.92±0.01 IU / ml。和温度。SDS-PAGE和粗酶的酶谱分析表明，两种菌株中CMCase的分子量均为60 kDa。与EF2和OW1-1的单培养相比，共培养的活性分别提高了15％和35.71％。菌株在更宽的温度和pH波动范围内具有较高的CMCase活性，可能是生物燃料行业的理想选择。