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Co-culturing of Novel Bacillus Species Isolated from Municipal Sludge and Gut of Red Wiggler Worm for Improving CMCase Activity

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Abstract

The novel bacterial strains EF2 and OW1-1 were isolated from intestine of Eisenia fetida and municipal organic waste respectively. The qualitative screening of strains in carboxymethyl cellulose (CMC) agar plate showed a larger zone of clearance with Gram’s iodine staining. The 16S rRNA gene sequences of the strains EF2 and OW1-1 were identified as a gram-positive Bacillus sp. The strains exhibited significantly high CMCase activities of 35.307 ± 0.08 IU/ml and 29.92 ± 0.01 IU/ml, respectively, in EF2 and OW1-1 when 2.5% (w/v) of lactose was used as a carbon source at their optimal pH and temperature. The SDS-PAGE and zymogram analysis of crude enzyme revealed that the molecular weight of CMCase was 60 kDa in both strains. In comparing to monoculture of EF2 and OW1-1, the co-culture increased their activity by 15% and 35.71% respectively. The higher CMCase activity of strains in a wider range of temperatures and pH fluctuation could be a good choice for biofuel industries.

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Funding

Funding was provided by BioFuelNet Canada (Project No. 67) and NSERC-RDF.

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  1. Department of Biology, Lakehead University, 955 Oliver Road, Thunder Bay, ON, P7B 5E1, Canada

    Hem Kanta Sharma & Wensheng Qin

  2. Department of Chemical and Biochemical Engineering, Western University, London, ON, N6A 5B9, Canada

    Chunbao Charles Xu

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Sharma, H.K., Xu, C.C. & Qin, W. Co-culturing of Novel Bacillus Species Isolated from Municipal Sludge and Gut of Red Wiggler Worm for Improving CMCase Activity. Waste Biomass Valor 11, 2047–2058 (2020). https://doi.org/10.1007/s12649-018-0448-x

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