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Determination of uric acid in blood
Reviews and Advances in Chemistry Pub Date : 2015-11-04 , DOI: 10.1134/s2079978015040019
V. A. Buzanovskii

The review presents a chronological development of methods to determine the uric acid concentration in plasma, serum, and whole blood, based on various analytical methods such as spectrophotometry, spectrofluorimetry, chromatography, gas chromatography–mass spectrometry, amperometry, and calorimetry. A brief overview of these methods and the results of comparative tests are given. It was noted that enzymatic and nonenzymatic spectrophotometric procedures to determine the uric acid concentration in plasma, serum, and whole blood are currently, the most widespread in small, medium, and large clinical diagnostic laboratories. In addition, a number of enzymatic spectrophotometric methods and chromatographic methods are used as expert procedures. Chromatography–mass spectrometry procedures are often applied to certify standard uric acid samples in blood serum. Enzymatic and nonenzymatic spectrofluorimetric procedures are mostly used in scientific biomedical research. Amperometric procedures to determine the uric acid concentration in blood are rarely used in modern clinical and diagnostic practice, and some nonenzymatic spectrophotometric and calorimetric procedures are actually not used.

中文翻译:

血液中尿酸的测定

综述基于各种分析方法,例如分光光度法,分光荧光法,色谱法,气相色谱-质谱法,安培法和量热法,提出了确定血浆,血清和全血中尿酸浓度的方法的按时间顺序发展的方法。简要概述了这些方法以及比较测试的结果。值得注意的是,目前在小型,中型和大型临床诊断实验室中,酶法和非酶法分光光度法可测定血浆,血清和全血中的尿酸浓度。另外,许多酶促分光光度法和色谱法被用作专家程序。色谱-质谱法通常用于验证血清中标准尿酸样品。酶促和非酶促荧光光谱法大多用于科学的生物医学研究。在现代临床和诊断实践中很少使用安培法测定血液中尿酸的浓度,实际上不使用某些非酶法分光光度法和量热法。
更新日期:2015-11-04
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