Skip to main content
SpringerLink
Log in

Determination of uric acid in blood

  • Published:
Review Journal of Chemistry Aims and scope Submit manuscript

Abstract

The review presents a chronological development of methods to determine the uric acid concentration in plasma, serum, and whole blood, based on various analytical methods such as spectrophotometry, spectrofluorimetry, chromatography, gas chromatography–mass spectrometry, amperometry, and calorimetry. A brief overview of these methods and the results of comparative tests are given. It was noted that enzymatic and nonenzymatic spectrophotometric procedures to determine the uric acid concentration in plasma, serum, and whole blood are currently, the most widespread in small, medium, and large clinical diagnostic laboratories. In addition, a number of enzymatic spectrophotometric methods and chromatographic methods are used as expert procedures. Chromatography–mass spectrometry procedures are often applied to certify standard uric acid samples in blood serum. Enzymatic and nonenzymatic spectrofluorimetric procedures are mostly used in scientific biomedical research. Amperometric procedures to determine the uric acid concentration in blood are rarely used in modern clinical and diagnostic practice, and some nonenzymatic spectrophotometric and calorimetric procedures are actually not used.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Archibald, R.M., Clin. Chem., 1957, vol. 3, no. 2, p. 102.

    CAS  Google Scholar 

  2. Alper, C. and Seitchik, J., Clin. Chem., 1957, vol. 3, no. 2, p. 95.

    CAS  Google Scholar 

  3. Kanabrocki, E.L., Greco, J., Wilkoff, L., and Veach, R., Clin. Chem., 1957, vol. 3, no. 3, p. 156.

    CAS  Google Scholar 

  4. Shapiro, B., Seligson, D., and Jessar, R., Clin. Chem., 1957, vol. 3, no. 3, p. 169.

    CAS  Google Scholar 

  5. Hausman, E.R., Lewis, G.T., and McAnally, J.S., Clin. Chem., 1957, vol. 3, no. 3, p. 657.

    CAS  Google Scholar 

  6. Sambhi, M.P. and Grollman, A., Clin. Chem., 1959, vol. 5, no. 6, p. 623.

    CAS  Google Scholar 

  7. London, M. and Marymont, J.H., Clin. Chem., 1964, vol. 10, no. 4, p. 298.

    CAS  Google Scholar 

  8. Crowley, L.V., Clin. Chem., 1964, vol. 10, no. 9, p. 838.

    CAS  Google Scholar 

  9. Marymont, J.H. and London, M., Clin. Chem., 1964, vol. 10, no. 10, p. 937.

    CAS  Google Scholar 

  10. O’Sullivan, J.B., Francis, J.O., and Kantor, N., Clin. Chem., 1965, vol. 11, no. 3, p. 427.

    Google Scholar 

  11. Caraway, W.T. and Marable, H., Clin. Chem., 1966, vol. 12, no. 1, p. 18.

    CAS  Google Scholar 

  12. Morgenstern, S., Flor, R.V., Kaufman, J.H., and Klein, B., Clin. Chem., 1966, vol. 12, no. 11, p. 748.

    CAS  Google Scholar 

  13. Nishi, H.H., Clin. Chem., 1967, vol. 13, no. 1, p. 12.

    CAS  Google Scholar 

  14. London, M. and Hums, M., Clin. Chem., 1967, vol. 13, no. 2, p. 132.

    CAS  Google Scholar 

  15. Kanter, S.L., Clin. Chem., 1967, vol. 13, no. 5, p. 406.

    CAS  Google Scholar 

  16. Klein, B., Clin. Chem., 1967, vol. 13, no. 5, p. 426.

    Google Scholar 

  17. Laessig, R.H., Underwood, C.E., and Basteyns, B.J., Clin. Chem., 1967, vol. 13, no. 11, p. 985.

    CAS  Google Scholar 

  18. Fales, F.W., Clin. Chem., 1968, vol. 14, no. 5, p. 449.

    CAS  Google Scholar 

  19. Wheat, J.L., Clin. Chem., 1968, vol. 14, no. 7, p. 630.

    CAS  Google Scholar 

  20. Patel, C.P., Clin. Chem., 1968, vol. 14, no. 8, p. 764.

    CAS  Google Scholar 

  21. Simkin, P.A., Clin. Chem., 1970, vol. 16, no. 3, p. 191.

    CAS  Google Scholar 

  22. Jung, D.H. and Parekh, A.C., Clin. Chem., 1970, vol. 16, no. 3, p. 247.

    CAS  Google Scholar 

  23. Carroll, J.J., Coburn, H., Douglass, R., and Babson, A.L., Clin. Chem., 1971, vol. 17, no. 3, p. 158.

    CAS  Google Scholar 

  24. Bell, R. and Ray, R.A., Clin. Chem., 1971, vol. 17, no. 7, p. 644.

    Google Scholar 

  25. Gochman, N. and Schmitz, J.M., Clin. Chem., 1971, vol. 17, no. 12, p. 1154.

    CAS  Google Scholar 

  26. Tiffany, T.O., Jansen, J.M., Burtis, C.A., Overton, J.B., and Scott, C.D., Clin. Chem., 1972, vol. 18, no. 8, p. 829.

    CAS  Google Scholar 

  27. Klein, B. and Lucas, L.B., Clin. Chem., 1973, vol. 19, no. 1, p. 67.

    CAS  Google Scholar 

  28. Kabasakalian, P., Kalliney, S., and Westcott, A., Clin. Chem., 1973, vol. 19, no. 5, p. 522.

    CAS  Google Scholar 

  29. Klein, B. and Sheehan, J., Clin. Chem., 1973, vol. 19, no. 5, p. 531.

    CAS  Google Scholar 

  30. Lum, G. and Gambino, S.R., Clin. Chem., 1973, vol. 19, no. 10, p. 1184.

    CAS  Google Scholar 

  31. Morin, L.G., Clin. Chem., 1974, vol. 20, no. 1, p. 51.

    CAS  Google Scholar 

  32. Meites, S., Thompson, C., and Roach, R.W., Clin. Chem., 1974, vol. 20, no. 7, p. 790.

    CAS  Google Scholar 

  33. Pesce, M.A., Bodourian, S.H., and Nicholson, J.F., Clin. Chem., 1974, vol. 20, no. 9, p. 1231.

    CAS  Google Scholar 

  34. Nelson, J.W. and Batra, K.K., Clin. Chem., 1975, vol. 21, no. 1, p. 125.

    CAS  Google Scholar 

  35. Moorehead, W.R. and Kasper, L.M., Clin. Chem., 1975, vol. 21, no. 4, p. 641.

    CAS  Google Scholar 

  36. Slaunwhite, W.D., Pachla, L.A., Wenke, D.C., and Kissinger, P.T., Clin. Chem., 1975, vol. 21, no. 10, p. 1427.

    CAS  Google Scholar 

  37. Cross, R.E., Heintges, M.G., Savory, J., and Wentz, P.W., Clin. Chem., 1976, vol. 22, no. 4, p. 429.

    CAS  Google Scholar 

  38. Kamoun, P., Lafourcade, G., and Jerome, H., Clin. Chem., 1976, vol. 22, no. 7, p. 964.

    CAS  Google Scholar 

  39. Rehak, N.N., Janes, G., and Young, D.S., Clin. Chem., 1977, vol. 23, no. 2, p. 195.

    CAS  Google Scholar 

  40. Liu, T.Z. and Khayam-Bashi, H., Clin. Chem., 1977, vol. 23, no. 3, p. 581.

    CAS  Google Scholar 

  41. White, R.M., Cross, R.E., and Savory, J., Clin. Chem., 1977, vol. 23, no. 9, p. 1538.

    CAS  Google Scholar 

  42. Robertson, E.A., Sliva, C.A., and Young, D.S., Clin. Chem., 1978, vol. 24, no. 1, p. 173.

    CAS  Google Scholar 

  43. Klose, S., Stoitz, M., Munz, E., and Portenhauser, R., Clin. Chem., 1978, vol. 24, no. 2, p. 250.

    CAS  Google Scholar 

  44. Kiser, E.J., Johnson, G.F., and Witte, D.L., Clin. Chem., 1978, vol. 24, no. 4, p. 536.

    CAS  Google Scholar 

  45. Trivedi, R.C., Rebar, L., Desai, K., and Stong, L.J., Clin. Chem., 1978, vol. 24, no. 4, p. 562.

    CAS  Google Scholar 

  46. Morris, J., Collins, P., and Fass, L., Clin. Chem., 1978, vol. 24, no. 7, p. 1289.

    CAS  Google Scholar 

  47. Lolekha, P.H., Chantarothipol, V., and Wongvibulsin, A., Clin. Chem., 1978, vol. 24, no. 9, p. 1578.

    CAS  Google Scholar 

  48. Sundaram, P.V., Igloi, M.P., Wassermann, R., and Hinsch, W., Clin. Chem., 1978, vol. 24, no. 10, p. 1813.

    CAS  Google Scholar 

  49. Haeckel, R., Clin. Chem., 1978, vol. 24, no. 10, p. 1846.

    CAS  Google Scholar 

  50. Trivedi, R.C., Rebar, L., Berta, E., and Stong, L., Clin. Chem., 1978, vol. 24, no. 11, p. 1908.

    CAS  Google Scholar 

  51. Kamoun, P. and Douay, O., Clin. Chem., 1978, vol. 24, no. 11, p. 2033.

    CAS  Google Scholar 

  52. Liu, T.Z., Clin. Chem., 1979, vol. 25, no. 2, p. 336.

    CAS  Google Scholar 

  53. Bartl, K., Brandhuber, M., and Ziegenhorn, J., Clin. Chem., 1979, vol. 25, no. 4, p. 619.

    CAS  Google Scholar 

  54. Mizon, J., Deroubaix, A., and Mizon-Capron, C., Clin. Chem., 1979, vol. 25, no. 6, p. 1184.

    CAS  Google Scholar 

  55. Neri, B., Clin. Chem., 1979, vol. 25, no. 6, p. 1185.

    CAS  Google Scholar 

  56. Hande, K.R., Perini, F., Putterman, G., and Elin, R., Clin. Chem., 1979, vol. 25, no. 8, p. 1492.

    CAS  Google Scholar 

  57. Chirillo, R., Caenaro, G., Pavan, B., and Pin, A., Clin. Chem., 1979, vol. 25, no. 10, p. 1744.

    CAS  Google Scholar 

  58. Pachla, L.A. and Kissinger, P.T., Clin. Chem., 1979, vol. 25, no. 10, p. 1847.

    CAS  Google Scholar 

  59. Zoppi, F., Fenili, D., Marcovina, S., and Peracino, A., Clin. Chem., 1980, vol. 26, no. 1, p. 178.

    CAS  Google Scholar 

  60. Fossati, P., Prencipe, L., and Berti, G., Clin. Chem., 1980, vol. 26, no. 2, p. 227.

    CAS  Google Scholar 

  61. Green, D.J. and Perlman, R.L., Clin. Chem., 1980, vol. 26, no. 6, p. 796.

    CAS  Google Scholar 

  62. Wung, W.E. and Howell, S.B., Clin. Chem., 1980, vol. 26, no. 12, p. 1704.

    CAS  Google Scholar 

  63. Bierer, D.W. and Quebbemann, A.J., Clin. Chem., 1981, vol. 27, no. 5, p. 756.

    Google Scholar 

  64. Liu, T.Z. and Khayam-Bashi, H., Clin. Chem., 1981, vol. 27, no. 6, p. 1144.

    CAS  Google Scholar 

  65. Duncan, P.H., Gochman, N., Cooper, T., Smith, E., and Bayse, D., Clin. Chem., 1982, vol. 28, no. 2, p. 284.

    CAS  Google Scholar 

  66. Duncan, P., Gochman, N., Bayse, D., Burnett, R., Carey, N., Carter, R., Fellows, W.D., Garber, C., Kessler, G., McComb, R., Miller, W., Nast, P., Ryan, W., Schaffer, R., Tejeda, B., Vanderlinde, R., and Widdowson, G., Clin. Chem., 1982, vol. 28, no. 2, p. 291.

    CAS  Google Scholar 

  67. Ingebretsen, O.C., Borgen, J., and Farstad, M., Clin. Chem., 1982, vol. 28, no. 3, p. 496.

    CAS  Google Scholar 

  68. Nanji, A.A., Clin. Chem., 1982, vol. 28, no. 5, p. 1242.

    CAS  Google Scholar 

  69. Shull, B.C. and Cerins-Overbey, N., Clin. Chem., 1982, vol. 28, no. 9, p. 1992.

    CAS  Google Scholar 

  70. Elin, R.J., Johnson, E., and Chesler, R., Clin. Chem., 1982, vol. 28, no. 10, p. 2098.

    CAS  Google Scholar 

  71. Mira, M., Stewart, P.M., Gebski, V., Liewellyn-Jones, D., and Abraham, S.F., Clin. Chem., 1984, vol. 30, no. 3, p. 380.

    CAS  Google Scholar 

  72. Masson, P. and Lindahl, I., Clin. Chem., 1984, vol. 30, no. 3, p. 493.

    CAS  Google Scholar 

  73. Davidson, D.C., Garfield, H.J., and Maney, F., Clin. Chem., 1984, vol. 30, no. 4, p. 584.

    CAS  Google Scholar 

  74. Dodds, J.M., Clin. Chem., 1984, vol. 30, no. 4, p. 585.

    CAS  Google Scholar 

  75. Kroll, M.H., Hagengruher, C., Lindsey, H., and Elin, R.J., Clin. Chem., 1984, vol. 30, no. 7, p. 1273.

    CAS  Google Scholar 

  76. Zhiri, A., Houot, O., Wellman-Bednawska, M., and Siest, G., Clin. Chem., 1985, vol. 31, no. 1, p. 109.

    CAS  Google Scholar 

  77. Merdes, H., Rittersdorf, W., and Werner, W., Clin. Chem., 1985, vol. 31, no. 6, p. 921.

    Google Scholar 

  78. Andranovich, T. and Kelner, M.J., Clin. Chem., 1986, vol. 32, no. 1, p. 177.

    CAS  Google Scholar 

  79. Dilena, B.A., Peake, M.J., Pardue, H.L., and Skoug, J.W., Clin. Chem., 1986, vol. 32, no. 3, p. 486.

    CAS  Google Scholar 

  80. Spain, M.A. and Wu, A.H.B., Clin. Chem., 1986, vol. 32, no. 3, p. 518.

    CAS  Google Scholar 

  81. Menon, R.K., Mikhallidis, D.P., Bell, J.L., Kernoff, P.B.A., and Dandona, P., Clin. Chem., 1986, vol. 32, no. 8, p. 1557.

    CAS  Google Scholar 

  82. Potter, J.L., Clin. Chem., 1987, vol. 33, no. 7, p. 1265.

    CAS  Google Scholar 

  83. Sakuma, R., Nishina, T., and Kitamura, M., Clin. Chem., 1987, vol. 33, no. 8, p. 1427.

    CAS  Google Scholar 

  84. Matheke, M.L., Kessler, G., and Chan, K.-M., Clin. Chem., 1987, vol. 33, no. 11, p. 2109.

  85. Cattozzo, G., Franzini, C., Hubbuch, A., and Tritschier, W., Clin. Chem., 1988, vol. 34, no. 2, p. 414.

    CAS  Google Scholar 

  86. Walker, F.B., Becker, D.M., Kowal-Neeley, B., and Krongaard, L.S., Clin. Chem., 1988, vol. 34, no. 5, p. 952.

    CAS  Google Scholar 

  87. Ellerbe, P., Cohen, A., Weich, M.J., and White, E., Clin. Chem., 1988, vol. 34, no. 11, p. 2280.

    CAS  Google Scholar 

  88. Tanaka, M. and Hama, M., Clin. Chem., 1988, vol. 34, no. 12, p. 2567.

    CAS  Google Scholar 

  89. Leary, E.T. and Taylor, S.G., Clin. Chem., 1988, vol. 34, no. 12, p. 2581.

    CAS  Google Scholar 

  90. Perez-Bendito, D., Gomez-Hens, A., Gutlerrez, M.C., and Anton, S., Clin. Chem., 1989, vol. 35, no. 2, pp. 230–233.

    CAS  Google Scholar 

  91. Wen-Fang, B., Ming-Quing, Y., Ke-Huai, P., Liang-Zhong, L., Fang-Hua, C., and Hua, L., Clin. Chem., 1991, vol. 37, no. 6, p. 1138.

    Google Scholar 

  92. Aoki, Y., Ihara, H., Nakamura, H., Aoki, T., and Yoshida, M., Clin. Chem., 1992, vol. 38, no. 7, p. 1350.

    CAS  Google Scholar 

  93. Stockl, D. and Reinauer, H., Clin. Chem., 1993, vol. 39, no. 6, p. 993.

    CAS  Google Scholar 

  94. Thienpont, L.M., De Leenheer, A.P., Stockl, D., and Reinauer, H., Clin. Chem., 1993, vol. 39, no. 6, p. 1001.

    CAS  Google Scholar 

  95. Beyne, P., Letteron, P., Herve, C., Roullet, J.-B., and Delacoux, E., Clin. Chem., 1996, vol. 42, no. 6, p. 988.

    CAS  Google Scholar 

  96. Lim, E., Bennett, P., and Beilby, J., Clin. Chem., 2003, vol. 49, no. 8, p. 1417.

    Article  CAS  Google Scholar 

  97. Xinhua, D., Clin. Chem., 2006, vol. 52, no. 11, p. 2117.

    Article  CAS  Google Scholar 

  98. Stove, V., Wuyts, B., and Delanghe, J., Clin. Chem., 2007, vol. 53, no. 2, p. 369.

    Article  CAS  Google Scholar 

  99. Koenig, W. and Meisinger, C., Clin. Chem., 2008, vol. 54, no. 2, p. 231.

    Article  CAS  Google Scholar 

  100. Strasak, A., Ruttmann, E., Brant, L., Kelleher, C., Klenk, J., Concin, H., Diem, G., Pfeiffer, K., Ulmer, H., and the VHM&PP Study Group, Clin. Chem., 2008, vol. 54, no. 2, p. 273.

    Article  CAS  Google Scholar 

  101. Chien, K.-L., Chen, M.-F., Hsu, H.-C., Chang, W.-T., Su, T.-C., Lee, Y.-T., and Hu, F.B., Clin. Chem., 2008, vol. 54, no. 2, p. 310.

    Article  CAS  Google Scholar 

  102. Zhang, W., Sun, K., Yang, Y., Zhang, H., Hu, F.B., and Hui, R., Clin. Chem., 2009, vol. 55, no. 11, p. 2026.

    Article  CAS  Google Scholar 

  103. Fossati, P. and Prencipe, L., Clin. Chem., 2010, vol. 56, no. 5, p. 865.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Kompaniya Bezopasnost’, Leninskii pr. 146, Moscow, 119526, Russia

    V. A. Buzanovskii

Authors
  1. V. A. Buzanovskii
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to V. A. Buzanovskii.

Additional information

Original Russian Text © V.A. Buzanovskii, 2015, published in Obzornyi Zhurnal po Khimii, 2015, Vol. 5, No. 4, pp. 315–364.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Buzanovskii, V.A. Determination of uric acid in blood. Ref. J. Chem. 5, 281–323 (2015). https://doi.org/10.1134/S2079978015040019

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S2079978015040019

Keywords

Search

Navigation