LATE ELONGATED HYPOCOTYL (LHY) gene is well known to be the core circadian function in Arabidopsis. In this study, DhLHY was obtained from Doritaenopsis ‘Tinny Tender’ (Doritaenopsis Happy smile × Happy valentine), and the sequence contained 2594 base pair (bp) in whole with an open reading frame (ORF) of 1962 bp encoding 653 amino acids. The putative protein of DhLHY included a HTH myb domain and a DNA binding site shared with the reported LHY family. Aligment of amino acid (aa) showed that DhLHY shared high similarity of LHY homologs from other species. Real-time quantitative reversed transcript polymerase chain reaction (qRT-PCR) analysis indicated that DhLHY was a morning-expressed circadian gene, and expressed greater in floral induction under low temperature. DhLHY was expressed both in vegetative and reproductive organs, and the highest level of its transcripts were in the pedicle and stem. Further functional study of DhLHY-overexpressed in Arobidopsis thaliana indicated the delayed bolting time and increased leaf length in transgenic plants. Results suggested that DhLHY participated in the circadian regulation and was response to the low temperature of floral induction in Doritaenopsis.

中文翻译：

---

DhLH​​Y，昼夜节律的Doritaenopsis杂种的表达基因，可促进低温下的花期转化，但在过度表达的转基因拟南芥中推迟开花

众所周知，晚期拟南芥（LHY）基因是拟南芥中的核心生物钟功能。在这项研究中，DhLH​​Y从获得Doritaenopsis “尖细投标”（Doritaenopsis快乐的微笑×情人节快乐），并且该序列包含在整个2594个碱基对（bp）的具有1962 bp的编码653个氨基酸的开放阅读框（ORF）。DhLH​​Y的推定蛋白包括HTH myb结构域和与报道的LHY家族共有的DNA结合位点。氨基酸（aa）的配对表明DhLHY与其他物种的LHY同源物具有高度相似性。实时定量逆转录聚合酶链反应（qRT-PCR）分析表明，DhLH​​Y是一个早上表达的昼夜节律基因，并在低温下以较高的花诱导表达。DhLH​​Y在营养和生殖器官中均有表达，其转录本的最高水平在椎弓根和茎中。在拟南芥中过表达的DhLH​​Y的进一步功能研究表明，转基因植物中的抽time时间延长且叶长增加。结果表明，DhLH​​Y参与昼夜节律调节和为响应于花诱导的在低温Doritaenopsis。