Abstract—The search for universal plant DNA barcodes has proved to be a big challenge. A single locus (or combination of several loci) that can be used for species identification has yet to be revealed, though the (rbcL + matK+ ITS) combination recommended in 2009 as the standard makes it possible to assign a species to the corresponding genus. The variability of some markers differs in different taxonomic groups and usually makes it possible to select the DNA barcode (sometimes even a mini-barcode) for a specific group, especially for applied tasks. Next-generation sequencing (NGS) methods make it possible to obtain a large number of extended DNA barcodes (sequences of complete chloroplast genomes and ribosomal genes), which allows the researcher to overcome the limitations of standard DNA barcodes. It is important that NGS technologies significantly enhance the possibility of the use of herbarium specimens. The search for plant DNA barcodes is ongoing.

中文翻译：

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植物DNA条形码

摘要—寻找通用的植物DNA条形码已被证明是一个巨大的挑战。可以用于物种识别的单个基因座（或几个基因座的组合）尚未揭示，尽管（rbcL + matK +ITS）组合在2009年被推荐为标准，因此可以将一个物种分配给相应的属。某些标记的变异性在不同的分类组中有所不同，通常可以为特定组（尤其是应用任务）选择DNA条形码（有时甚至是微型条形码）。下一代测序（NGS）方法使获得大量扩展的DNA条形码（完整的叶绿体基因组和核糖体基因的序列）成为可能，这使研究人员能够克服标准DNA条形码的局限性。NGS技术显着提高使用标本室标本的可能性，这一点很重要。正在寻找植物DNA条形码。