Salmonella enterica serovar Typhimurium (S. Typhimurium) is an important gram-negative intracellular pathogen that infects humans and animals. More than 50 putative regulatory proteins have been identified in the S. Typhimurium genome, but few have been clearly defined. In this study, the physiological function and regulatory role of STM14_3563, which encodes a ParD family putative transcriptional regulator in S. Typhimurium, were investigated. Macrophage replication assays and mice experiments revealed that S. Typhimurium showed reduced growth in murine macrophages and attenuated virulence in mice owing to deletion of STM14_3563 gene. RNA sequencing (RNA-Seq) data showed that STM14_3563 exerts wide-ranging effects on gene expression in S. Typhimurium. STM14_3563 activates the expression of several genes encoded in Salmonella pathogenicity island (SPI)-6, SPI-12, and SPI-13, which are required for intracellular replication of S. Typhimurium. Additionally, the global transcriptional regulator Fis was found to directly activate STM14_3563 expression by binding to the STM14_3563 promoter. These results indicate that STM14_3563 is involved in the regulation of a variety of virulence-related genes in S. Typhimurium that contribute to its growth in macrophages and virulence in mice.

中文翻译：

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假定的转录调节因子STM14_3563通过激活毒力相关基因来促进鼠伤寒沙门氏菌的致病性。

肠炎沙门氏菌鼠伤寒沙门氏菌（S. Typhimurium）是一种重要的革兰氏阴性细胞内病原体，可感染人类和动物。超过50个假定调控蛋白已在已确定S.鼠伤寒沙门氏菌的基因组，但很少有明确的规定。在这项研究中，生理功能和调节作用STM14_3563，这在编码PARD家庭假定转录调节小号。鼠伤寒，进行了调查。巨噬细胞复制测定和小鼠的实验显示，S.鼠伤寒沙门氏菌显示出在小鼠巨噬细胞减少的生长和衰减由于缺失小鼠中的毒力STM14_3563基因。RNA测序（RNA-Seq）数据表明，STM14_3563对S中的基因表达具有广泛的影响。鼠伤寒 STM14_3563激活沙门氏菌致病岛（SPI）-6，SPI-12和SPI-13中编码的几个基因的表达，这些基因是S的细胞内复制所必需的。鼠伤寒 另外，发现全局转录调节子Fis通过与STM14_3563启动子结合而直接激活STM14_3563表达。这些结果表明，STM14_3563参与多种毒力相关的基因的调节S.鼠伤寒沙门氏菌，有助于其在巨噬细胞的生长和毒力的小鼠。