In this study, we analysed the impact of population size and isolation on the genetic variation of the short-lived alpine plant species Gentianella campestris ssp. campestris from two study regions (Allgäu and Karwendel) in the Northern calcareous Alps in Germany. We determined the size and isolation of the study populations and analysed genetic variation using amplified fragment length polymorphisms. Genetic variation of G. campestris ssp. campestris differed significantly between the two study regions. Genetic variation did not depend on population size. However, the level of genetic variation within populations was about three times lower in the Karwendel, where the species is much more isolated than in the Allgäu. Conversely, genetic variation among populations was much stronger in the Karwendel than in the Allgäu. Our results support the observation that the level of genetic variation within populations of alpine plant species may not only be affected by population size, but also by population isolation. Depending on the distance among populations, gene flow by exchange of pollen and seeds triggers the influx of genetic variation, thereby sometimes superimposing the effects of population size. Our results suggest that for seed collections in conservation projects, not only population size, but also isolation should be considered.

中文翻译：

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Gentianella campestris ssp。的遗传变异。北阿尔卑斯地区的樟树：人口规模和孤立性有多重要？

在这项研究中，我们分析了种群数量和隔离对短命高山植物Gentianella campestris ssp遗传变异的影响。油菜从两个研究区（阿尔及的Karwendel）在北阿尔卑斯山的石灰质在德国。我们确定了研究人群的大小和隔离，并使用扩增的片段长度多态性分析了遗传变异。G. campestris ssp。的遗传变异。桔梗两个研究区域之间存在显着差异。遗传变异不取决于种群数量。但是，Karwendel种群内的遗传变异水平要低大约三倍，那里的物种比Allgäu更为孤立。相反，Karwendel人群中的遗传变异比Allgäu强烈得多。我们的结果支持以下观察：高山植物物种种群内的遗传变异水平不仅可能受到种群数量的影响，而且还可能受到种群隔离的影响。根据种群之间的距离，通过交换花粉和种子而产生的基因流会触发遗传变异的涌入，从而有时会叠加种群数量的影响。我们的结果表明，对于保护项目中的种子收集，不仅是种群数量，