To date studies revealed that MYB28 intensively involved biosynthesis of GSLs, there is no direct evidence of MYB28 in glucoraphanin (GR) biosynthesis of broccoli. Furthermore, Agrobacterium (Agro)-infiltration also induced GSLs in Arabidopsis. However, there is no information regarding Agro-dependent GR accumulation in broccoli. We revealed that over-expression of BroMYB28 could accumulate GR, and Agro-infiltration is also enough to induce the accumulation of GR in broccoli. From our results, it is suggested that they used different regulatory mechanisms; BroMYB28 over-expression induced both BroMYB28 and BroMYB29 (Bol008849-like) transcripts; Agro-infiltration induced only BroMYB29 (Bol008849-like) transcripts.

中文翻译：

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农杆菌浸润和BroMYB28瞬时过表达对西兰花叶片中糖尿素生物合成的影响

迄今为止的研究表明，MYB28紧密参与了GSL的生物合成，在花椰菜糖苷（GR）的生物合成中没有MYB28的直接证据。此外，农杆菌（Agro）-浸润还诱导了拟南芥中的GSL 。但是，没有关于花椰菜中农业依赖性GR积累的信息。我们发现，BroMYB28的过表达可以积累GR，而农杆菌浸润也足以诱导西兰花中GR的积累。根据我们的结果，建议他们使用不同的监管机制。BroMYB28过表达诱导两者BroMYB28和BroMYB29（Bol008849 - like）转录本; 农业-infiltration仅诱导BroMYB29（Bol008849 -像）成绩单。