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Impact of Agrobacterium-infiltration and transient overexpression of BroMYB28 on glucoraphanin biosynthesis in broccoli leaves

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Abstract

To date studies revealed that MYB28 intensively involved biosynthesis of GSLs, there is no direct evidence of MYB28 in glucoraphanin (GR) biosynthesis of broccoli. Furthermore, Agrobacterium (Agro)-infiltration also induced GSLs in Arabidopsis. However, there is no information regarding Agro-dependent GR accumulation in broccoli. We revealed that over-expression of BroMYB28 could accumulate GR, and Agro-infiltration is also enough to induce the accumulation of GR in broccoli. From our results, it is suggested that they used different regulatory mechanisms; BroMYB28 over-expression induced both BroMYB28 and BroMYB29 (Bol008849-like) transcripts; Agro-infiltration induced only BroMYB29 (Bol008849-like) transcripts.

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Acknowledgements

This work was supported by grants from the Bio-industry Technology Development Program (Grant nos. 117043-3, 111057-5) of iPET (Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry).

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  1. Young-Cheon Kim and Ahra Cha equally contributed in this work.

Authors and Affiliations

  1. Department of Bioindustry and Bioresource Engineering, Sejong University, Seoul, 05006, Korea

    Young-Cheon Kim, Ahra Cha, Muhammad Hussain & Sanghyeob Lee

  2. Department Biology, Ludwig-Maximilians-University Munich, 82152, Martinsried, Germany

    Kwanuk Lee

  3. Plant Engineering Research Institute, Sejong University, Seoul, 05006, Korea

    Sanghyeob Lee

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  1. Young-Cheon Kim
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  2. Ahra Cha
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  3. Muhammad Hussain
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Correspondence to Sanghyeob Lee.

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Kim, YC., Cha, A., Hussain, M. et al. Impact of Agrobacterium-infiltration and transient overexpression of BroMYB28 on glucoraphanin biosynthesis in broccoli leaves. Plant Biotechnol Rep 14, 373–380 (2020). https://doi.org/10.1007/s11816-019-00591-8

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