In this study, α-TCP and β-TCP were successfully produced via a mechanochemical method from aragonite structures, Clinocardium ciliatum seashells, at 850 °C, 1000 °C, and 1200 °C sintering temperatures. The characterization of the obtained materials was carried out via FT-IR, SEM/EDX, BET, XRD, ICP-OES analysis. Samples were soaked for 21 days in simulated body fluid (SBF) for a bioactivity test. Additionally, MTT assay was applied to determine the cell viability of samples. Bioactivity in vitro tests showed that bone-like hydroxyapatite formed when the α-TCP and β-TCP were soaked in SBF. TCP bioceramics had a noncytotoxicity effect on SAOS-2 osteoblast-like cells and cell viability increased in 1 to 7 days. The produced bioceramics have ideal pore sizes and properties that are suitable for supporting biological activities. Tris-HCl buffer solution was used to obtain the level of biodegradation. It was seen that α-TCP exhibited better dissolution features than β-TCP.

中文翻译：

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纤毛心形贝壳产生的α-TCP和β-TCP的体外研究

在这项研究中，通过机械化学方法成功地从文石结构纤毛斜柏中产生了α-TCP和β-TCP贝壳在850°C，1000°C和1200°C的烧结温度下工作。通过FT-IR，SEM / EDX，BET，XRD，ICP-OES分析对获得的材料进行表征。将样品在模拟体液（SBF）中浸泡21天以进行生物活性测试。另外，应用MTT测定法确定样品的细胞活力。体外生物活性测试表明，将α-TCP和β-TCP浸入SBF中会形成骨状羟基磷灰石。TCP生物陶瓷对SAOS-2成骨细胞样细胞具有非细胞毒性作用，并且细胞活力在1至7天内增加。所生产的生物陶瓷具有理想的孔径和性能，适合于支持生物活性。使用Tris-HCl缓冲溶液获得生物降解水平。可以看出，α-TCP表现出比β-TCP更好的溶解特性。