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In vitro studies of α-TCP and β-TCP produced from Clinocardium ciliatum seashells

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Abstract

In this study, α-TCP and β-TCP were successfully produced via a mechanochemical method from aragonite structures, Clinocardium ciliatum seashells, at 850 °C, 1000 °C, and 1200 °C sintering temperatures. The characterization of the obtained materials was carried out via FT-IR, SEM/EDX, BET, XRD, ICP-OES analysis. Samples were soaked for 21 days in simulated body fluid (SBF) for a bioactivity test. Additionally, MTT assay was applied to determine the cell viability of samples. Bioactivity in vitro tests showed that bone-like hydroxyapatite formed when the α-TCP and β-TCP were soaked in SBF. TCP bioceramics had a noncytotoxicity effect on SAOS-2 osteoblast-like cells and cell viability increased in 1 to 7 days. The produced bioceramics have ideal pore sizes and properties that are suitable for supporting biological activities. Tris-HCl buffer solution was used to obtain the level of biodegradation. It was seen that α-TCP exhibited better dissolution features than β-TCP.

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Acknowledgments

Special thanks to ArelPOTKAM (Polymer Technologies and Composite Application and Research Center) where the synthesis of bioceramics took place.

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Authors and Affiliations

  1. ArelPOTKAM (Polymer Technologies and Composite Application and Research Center), Istanbul Arel University, Istanbul, Turkey

    Yesim Muge Sahin

  2. Department of Biomedical Engineering, Istanbul Arel University, Istanbul, Turkey

    Yesim Muge Sahin

  3. Department of Bioengineering, Yildiz Technical University, Istanbul, Turkey

    Zeynep Orman & Sevil Yucel

Authors
  1. Yesim Muge Sahin
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  2. Zeynep Orman
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Correspondence to Yesim Muge Sahin.

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Sahin, Y.M., Orman, Z. & Yucel, S. In vitro studies of α-TCP and β-TCP produced from Clinocardium ciliatum seashells. J Aust Ceram Soc 56, 477–488 (2020). https://doi.org/10.1007/s41779-019-00355-1

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