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Differential metabolism of neonicotinoids by brown planthopper, Nilaparvata lugens, CYP6ER1 variants
Pesticide Biochemistry and Physiology ( IF 4.2 ) Pub Date : 2020-05-01 , DOI: 10.1016/j.pestbp.2020.02.004 Akira Hamada 1 , Lynn Stam 2 , Toshifumi Nakao 1 , Miyuki Kawashima 1 , Shinichi Banba 1
Pesticide Biochemistry and Physiology ( IF 4.2 ) Pub Date : 2020-05-01 , DOI: 10.1016/j.pestbp.2020.02.004 Akira Hamada 1 , Lynn Stam 2 , Toshifumi Nakao 1 , Miyuki Kawashima 1 , Shinichi Banba 1
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Imidacloprid is very effective in controlling Nilaparvata lugens Stål, which severely damages rice plants. Following heavy imidacloprid use, imidacloprid-resistant N. lugens, which showed cross-resistance to other neonicotinoids, appeared. We used the baculovirus/Sf9 expression system to express CYP6ER1 variants carrying A375del + A376G (del3) mutations, either with or without T318S mutation, which confer imidacloprid resistance in N. lugens. These CYP6ER1 variants metabolized imidacloprid but did not metabolize dinotefuran. Moreover, Drosophila expressing a CYP6ER1 variant carrying T318S + del3 mutations were resistant to imidacloprid, with a resistance ratio of 288.7, whereas the resistance ratio to dinotefuran was 3.6. These findings indicate that N. lugens has a low level of resistance to dinotefuran, and the increase of resistance is slow. We also studied the metabolism of other neonicotinoids, as well as sulfoxaflor and flupyradifurone, by CYP6ER1 variants carrying del3 mutations, either with or without the T318S mutation. Sulfoxaflor, was not metabolized by either CYP6ER1-del3 or CYP6ER1-T318Sdel3 variants. However, these variants did metabolize flupyradifurone. This study sheds light on the substrate selectivity of CYP6ER1 variants.
中文翻译：
褐飞虱、褐飞虱、CYP6ER1 变体对新烟碱类物质的差异代谢
吡虫啉对控制对水稻植株造成严重危害的褐飞虱非常有效。在大量使用吡虫啉后，出现了对其他新烟碱类药物具有交叉抗性的抗吡虫啉的褐飞虱。我们使用杆状病毒/Sf9 表达系统来表达携带 A375del + A376G (del3) 突变的 CYP6ER1 变体，无论是否有 T318S 突变，这在 N. lugens 中赋予了吡虫啉抗性。这些 CYP6ER1 变体代谢吡虫啉但不代谢呋虫胺。此外，表达携带 T318S + del3 突变的 CYP6ER1 变体的果蝇对吡虫啉具有抗性，抗性比为 288.7，而对呋虫胺的抗性比为 3.6。这些发现表明，褐飞虱对呋虫胺的抗性水平较低，抗性增加缓慢。我们还通过携带 del3 突变的 CYP6ER1 变体（有或没有 T318S 突变）研究了其他新烟碱类物质以及氟啶虫胺和氟吡呋酮的代谢。磺胺嘧啶不被 CYP6ER1-del3 或 CYP6ER1-T318Sdel3 变体代谢。然而，这些变体确实代谢了氟吡呋酮。该研究阐明了 CYP6ER1 变体的底物选择性。
更新日期：2020-05-01
中文翻译：
褐飞虱、褐飞虱、CYP6ER1 变体对新烟碱类物质的差异代谢
吡虫啉对控制对水稻植株造成严重危害的褐飞虱非常有效。在大量使用吡虫啉后，出现了对其他新烟碱类药物具有交叉抗性的抗吡虫啉的褐飞虱。我们使用杆状病毒/Sf9 表达系统来表达携带 A375del + A376G (del3) 突变的 CYP6ER1 变体，无论是否有 T318S 突变，这在 N. lugens 中赋予了吡虫啉抗性。这些 CYP6ER1 变体代谢吡虫啉但不代谢呋虫胺。此外，表达携带 T318S + del3 突变的 CYP6ER1 变体的果蝇对吡虫啉具有抗性，抗性比为 288.7，而对呋虫胺的抗性比为 3.6。这些发现表明，褐飞虱对呋虫胺的抗性水平较低，抗性增加缓慢。我们还通过携带 del3 突变的 CYP6ER1 变体（有或没有 T318S 突变）研究了其他新烟碱类物质以及氟啶虫胺和氟吡呋酮的代谢。磺胺嘧啶不被 CYP6ER1-del3 或 CYP6ER1-T318Sdel3 变体代谢。然而，这些变体确实代谢了氟吡呋酮。该研究阐明了 CYP6ER1 变体的底物选择性。
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