The structure of the cotton uceA1.7 promoter and its modules was analyzed; the potential of their key sequences has been confirmed in different tissues, proving to be a good candidate for the development of new biotechnological tools. Transcriptional promoters are among the primary genetic engineering elements used to control genes of interest (GOIs) associated with agronomic traits. Cotton uceA1.7 was previously characterized as a constitutive promoter with activity higher than that of the constitutive promoter from the Cauliflower mosaic virus (CaMV) 35S gene in various plant tissues. In this study, we generated Arabidopsis thaliana homozygous events stably overexpressing the gfp reporter gene driven by different modules of the uceA1.7 promoter. The expression level of the reporter gene in different plant tissues and the transcriptional stability of these modules was determined compared to its full-length promoter and the 35S promoter. The full-length uceA1.7 promoter exhibited higher activity in different plant tissues compared to the 35S promoter. Two modules of the promoter produced a low and unstable transcription level compared to the other promoters. The other two modules rich in cis-regulatory elements showed similar activity levels to full-length uceA1.7 and 35S promoters but were less stable. This result suggests the location of a minimal portion of the promoter that is required to initiate transcription properly (the core promoter). Additionally, the full-length uceA1.7 promoter containing the 5′-untranslated region (UTR) is essential for higher transcriptional stability in various plant tissues. These findings confirm the potential use of the full-length uceA1.7 promoter for the development of new biotechnological tools (NBTs) to achieve higher expression levels of GOIs in, for example, the root or flower bud for the efficient control of phytonematodes and pest-insects, respectively, in important crops.

中文翻译：

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使用棉花 uceA1.7 启动子的不同模块获得的见解

分析了棉花uceA1.7启动子及其模块的结构；它们的关键序列的潜力已在不同组织中得到证实，被证明是开发新生物技术工具的良好候选者。转录启动子是用于控制与农艺性状相关的感兴趣基因 (GOI) 的主要基因工程元件之一。棉花 uceA1.7 以前被表征为组成型启动子，其活性高于各种植物组织中花椰菜花叶病毒 (CaMV) 35S 基因的组成型启动子。在这项研究中，我们产生了稳定过表达由 uceA1.7 启动子的不同模块驱动的 gfp 报告基因的拟南芥纯合事件。与其全长启动子和 35S 启动子相比，确定了报告基因在不同植物组织中的表达水平和这些模块的转录稳定性。与 35S 启动子相比，全长 uceA1.7 启动子在不同植物组织中表现出更高的活性。与其他启动子相比，启动子的两个模块产生了低且不稳定的转录水平。其他两个富含顺式调节元件的模块显示出与全长 uceA1.7 和 35S 启动子相似的活性水平，但稳定性较差。该结果表明正确启动转录所需的启动子的最小部分（核心启动子）的位置。此外，全长 uceA1。含有 5'-非翻译区 (UTR) 的 7 启动子对于各种植物组织中更高的转录稳定性至关重要。这些发现证实了全长 uceA1.7 启动子在开发新的生物技术工具 (NBT) 以实现 GOI 在例如根或花蕾中的更高表达水平的潜在用途，以有效控制植物线虫和害虫- 昆虫，分别在重要作物中。