Efficient analytical methods are required for optimizing dosage of veterinary antibiotics and hormones in order to reduce toxicity and antimicrobial resistance in the environment. Thus, the objective of this work was to develop a rapid and low-cost method for determination of hormone estradiol and antibiotic chlortetracycline in bovine and porcine blood serum by aqueous two-phase system (ATPS) extraction and capillary electrophoresis quantification. ATPS based on ionic liquid cholinium alaninate and citrate salt along with mixtures of protic and aprotic polar solvents were evaluated in terms of recovery of extraction (%R). The liquid-liquid equilibrium, phase diagrams, and tie lines are discussed. Antibiotic migrated to solvent-rich phase (R ≈ 89.0%) to all systems. Estradiol migrates to ionic liquid-rich phase; however, addition of 10% methanol changed partition to solvent-rich phase (R ≈ 89.7%). The method has high recovery and cleanliness, is cost-efficient, scalable, and hence is adequate for screening of antibiotics and hormones tested in animal blood serum for dosage optimization and to predict their environment.

中文翻译：

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使用水性两相系统作为环境影响的预测因子，从动物血清中同时提取抗生素和雌激素。

需要有效的分析方法来优化兽用抗生素和激素的用量，以减少环境中的毒性和抗药性。因此，这项工作的目的是开发一种快速低成本的方法，通过水两相系统（ATPS）萃取和毛细管电泳定量测定牛和猪血清中的激素雌二醇和抗生素金霉素。根据萃取回收率（％R）评估了基于离子液体丙氨酸胆碱盐和柠檬酸盐以及质子和非质子极性溶剂混合物的ATPS。讨论了液-液平衡，相图和联系线。抗生素迁移到所有系统的溶剂富集相（R≈89.0％）。雌二醇迁移至离子液体富集相；然而，加入10％甲醇会改变分配到富溶剂相的分配（R≈89.7％）。该方法具有较高的回收率和清洁度，具有成本效益，可扩展性，因此足以筛选动物血清中测试的抗生素和激素，以优化剂量并预测其环境。