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Simultaneous extraction of antibiotic and estrogen from animal blood serum using aqueous two-phase systems as predictor of environmental impact

  • Advances in Environmental Biotechnology and Engineering 2018
  • Published:
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Abstract

Efficient analytical methods are required for optimizing dosage of veterinary antibiotics and hormones in order to reduce toxicity and antimicrobial resistance in the environment. Thus, the objective of this work was to develop a rapid and low-cost method for determination of hormone estradiol and antibiotic chlortetracycline in bovine and porcine blood serum by aqueous two-phase system (ATPS) extraction and capillary electrophoresis quantification. ATPS based on ionic liquid cholinium alaninate and citrate salt along with mixtures of protic and aprotic polar solvents were evaluated in terms of recovery of extraction (%R). The liquid-liquid equilibrium, phase diagrams, and tie lines are discussed. Antibiotic migrated to solvent-rich phase (R ≈ 89.0%) to all systems. Estradiol migrates to ionic liquid-rich phase; however, addition of 10% methanol changed partition to solvent-rich phase (R ≈ 89.7%). The method has high recovery and cleanliness, is cost-efficient, scalable, and hence is adequate for screening of antibiotics and hormones tested in animal blood serum for dosage optimization and to predict their environment.

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Funding

We appreciate the financing support of Instituto Tecnológico de Sonora through the PROFAPI program (PROFAPI 2019_0026); C. Díaz would like to acknowledge the scholarship from CONACYT.

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Authors and Affiliations

  1. Department of Biotechnology and Food Science, Technological Institute of Sonora, 85130, Ciudad Obregón, Mexico

    Carlos Díaz-Quiroz, Gabriela Ulloa-Mercado & Pablo Gortáres-Moroyoqui

  2. Department of Agronomic Sciences and Veterinary, Technological Institute of Sonora, 85130, Ciudad Obregón, Mexico

    Juan Francisco Hernández-Chávez & Ramón Miguel Molina-Barrios

  3. Department of Chemical Engineering, University of Vigo, 36310, Vigo, Spain

    Francisco Deive

Authors
  1. Carlos Díaz-Quiroz
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  2. Juan Francisco Hernández-Chávez
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  3. Gabriela Ulloa-Mercado
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  4. Francisco Deive
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  5. Pablo Gortáres-Moroyoqui
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  6. Ramón Miguel Molina-Barrios
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Corresponding authors

Correspondence to Juan Francisco Hernández-Chávez or Gabriela Ulloa-Mercado.

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Responsible editor: Ester Heath

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Díaz-Quiroz, C., Hernández-Chávez, J.F., Ulloa-Mercado, G. et al. Simultaneous extraction of antibiotic and estrogen from animal blood serum using aqueous two-phase systems as predictor of environmental impact. Environ Sci Pollut Res 27, 28536–28544 (2020). https://doi.org/10.1007/s11356-020-07770-z

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  • DOI: https://doi.org/10.1007/s11356-020-07770-z

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