Glycine soja germplasm can be used to successfully introduce new alleles with the potential to add valuable new genetic diversity to the current elite soybean gene pool. Given the demonstrated narrow genetic base of the US soybean production, it is essential to identify beneficial alleles from exotic germplasm, such as wild soybean, to enhance genetic gain for favorable traits. Nested association mapping (NAM) is an approach to population development that permits the comparison of allelic effects of the same QTL in multiple parents. Seed yield, plant maturity, plant height and plant lodging were evaluated in a NAM panel consisting of 392 recombinant inbred lines derived from three biparental interspecific soybean populations in eight environments during 2016 and 2017. Nested association mapping, combined with linkage mapping, identified three major QTL for plant maturity in chromosomes 6, 11 and 12 associated with alleles from wild soybean resulting in significant increases in days to maturity. A significant QTL for plant height was identified on chromosome 13 with the allele increasing plant height derived from wild soybean. A significant grain yield QTL was detected on chromosome 17, and the allele from Glycine soja had a positive effect of 166 kg ha-1; RIL's with the wild soybean allele yielded on average 6% more than the lines carrying the Glycine max allele. These findings demonstrate the usefulness and potential of alleles from wild soybean germplasm to enhance important agronomic traits in a soybean breeding program.

中文翻译：

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三个种间大豆群体重要农艺性状的嵌套关联作图。


大豆种质可用于成功引入新的等位基因，有可能为当前的优良大豆基因库添加有价值的新遗传多样性。鉴于美国大豆生产的遗传基础较窄，有必要从野生大豆等外来种质中鉴定有益的等位基因，以增强有利性状的遗传增益。嵌套关联作图 (NAM) 是一种种群发展方法，可以比较多个亲本中相同 QTL 的等位基因效应。在 NAM 组中评估了种子产量、植物成熟度、植物高度和植物倒伏，该组由 392 个重组自交系组成，这些自交系源自 2016 年和 2017 年八个环境中的三个双亲种间大豆群体。嵌套关联作图与连锁作图相结合，确定了三个主要6、11 和 12 号染色体中植物成熟的 QTL 与野生大豆的等位基因相关，导致成熟天数显着增加。在 13 号染色体上鉴定出一个显着的株高 QTL，该等位基因增加了源自野生大豆的株高。在17号染色​​体上检测到显着的籽粒产量QTL，来自大豆的等位基因具有166公斤ha-1的正效应；带有野生大豆等位基因的 RIL 品系比带有 Glycine max 等位基因的品系平均产量高 6%。这些发现证明了野生大豆种质等位基因在增强大豆育种计划中重要农艺性状方面的有用性和潜力。