Papillomaviruses infect the skin and mucosal surfaces of diverse animal hosts with consequences ranging from asymptomatic colonization to highly malignant epithelial cancers. Increasing evidence suggests a role for papillomaviruses in the most common cutaneous malignancy of domestic cats, squamous cell carcinoma (SCC). Using total DNA sequencing we identified a novel feline papillomavirus in a nasal biopsy taken from a cat presenting with both nasal cavity lymphoma and recurrent squamous cell carcinoma affecting the nasal planum. We designate this novel virus as Felis catus papillomavirus 6 (FcaPV6). The complete FcaPV6 7453 bp genome was similar to those of other feline papillomaviruses and phylogenetic analysis revealed that it was most closely related to FcaPV3, although was distinct enough to represent a new viral type. Classification of FcaPV6 in a new genus alongside FcaPVs 3, 4 and 5 is supported. Archived excisional biopsy of the SCC, taken 20 months prior to presentation, was intensely positive on p16 immunostaining. FcaPV6, amplified using virus-specific, but not consensus, PCR, was the only papillomavirus detected in DNA extracted from the SCC. Conversely, renal lymphoma, sampled at necropsy two months after presentation, tested negative on FcaPV6-specific PCR. In sum, using metagenomics we demonstrate the presence of a novel feline papillomavirus in association with cutaneous squamous cell carcinoma.

中文翻译：

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新型猫乳头瘤病毒与鳞状细胞癌相关的家猫的鉴定。

乳头瘤病毒感染各种动物宿主的皮肤和粘膜表面，其后果从无症状定植到高度恶性上皮癌不等。越来越多的证据表明，乳头瘤病毒在家猫最常见的皮肤恶性肿瘤鳞状细胞癌（SCC）中起着作用。使用总DNA测序，我们在取自有鼻腔淋巴瘤和复发性鼻鳞癌的猫的鼻活检中鉴定了一种新型猫乳头瘤病毒。我们将此新型病毒命名为Felis catus乳头瘤病毒6（FcaPV6）。完整的FcaPV6 7453 bp基因组与其他猫乳头瘤病毒的基因组相似，并且系统发育分析表明，它与FcaPV3的关系最为密切，尽管其区别足以代表一种新型病毒。支持将FcaPV6与FcaPV 3、4和5一起归类。呈递前20个月进行的SCC存档切除活检对p16免疫染色强烈阳性。FcaPV6是使用病毒特异性但非共识的PCR扩增的，是从SCC提取的DNA中唯一检测到的乳头瘤病毒。相反，在出现后两个月的尸检中取样的肾淋巴瘤在FcaPV6特异性PCR上呈阴性。总之，使用宏基因组学，我们证明了新型猫乳头瘤病毒与皮肤鳞状细胞癌有关。是从SCC提取的DNA中唯一检测到的乳头瘤病毒。相反，在出现后两个月的尸检中取样的肾淋巴瘤在FcaPV6特异性PCR上呈阴性。总之，使用宏基因组学，我们证明了新型猫乳头瘤病毒与皮肤鳞状细胞癌有关。是从SCC提取的DNA中唯一检测到的乳头瘤病毒。相反，在出现后两个月的尸检中取样的肾淋巴瘤在FcaPV6特异性PCR上呈阴性。总之，使用宏基因组学，我们证明了新型猫乳头瘤病毒与皮肤鳞状细胞癌有关。