Aim

The objective of this study was to evaluate the presence of microorganisms in the root canals (RC) of teeth submitted to endodontic retreatment due to prosthetic reasons and without evidence of apical periodontitis.

Material and methods

Twenty teeth referred to endodontic retreatment due to prosthetic reasons and without evidence of apical periodontitis were included in this study. Gutta-percha (GP) was collected from each third of the RC. After GP removal, microbial samples were also collected from the full extension of RC using paper points and files. The samples were cultivated in Fastidious Anaerobe Agar and the colony-forming units (CFU/mL) were counted. They were also semi-quantitatively analyzed by checkerboard DNA–DNA hybridization using 40 DNA bacterial probes.

Results

Microorganisms were found in all samples. The coronal third of GP was more contaminated than the apical third (p ≤ 0.05). There was prevalence of Enterococcus hirae and Enterococcus faecalis in all RC thirds and also in the samples collected from the full extension of RCs.

Conclusion

Microorganisms were found in all cases referred to endodontic retreatment due to prosthetic reasons and without evidence of apical periodontitis. Enterococcus was the genus most frequently detected.

Clinical significance

An endodontic retreatment should be considered before replacing a prosthesis.

中文翻译：

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评估由于修复原因而没有进行根尖性牙周炎的证据而需要接受再治疗的牙齿根管中微生物的存在。

目标

这项研究的目的是评估由于假体原因并且没有根尖性牙周炎的证据，接受牙髓治疗的牙齿的根管（RC）中是否存在微生物。

材料与方法

由于假体原因并且没有根尖性牙周炎的证据，有20颗牙齿被称为牙髓治疗。从RC的三分之一收集古塔胶（GP）。去除GP后，还使用纸点和文件从RC的整个扩展部分收集了微生物样品。将样品在Fastidious Anaerobe琼脂中培养，并计算菌落形成单位（CFU / mL）。还使用40个DNA细菌探针通过棋盘DNA-DNA杂交对它们进行了半定量分析。

结果

在所有样品中均发现了微生物。GP的冠第三比心尖第三（被更污染p  ≤0.05）。有患病肠球菌和粪肠球菌中的所有RC三分之二和驻地协调员的全面推广采集的样品也英寸

结论

在所有因假体原因而没有根尖性牙周炎的证据的情况下，均发现需要进行牙髓治疗的微生物。肠球菌是最常被发现的属。

临床意义

在更换假体之前，应考虑进行牙髓治疗。