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Evaluation of the presence of microorganisms from root canal of teeth submitted to retreatment due to prosthetic reasons and without evidence of apical periodontitis

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Abstract

Aim

The objective of this study was to evaluate the presence of microorganisms in the root canals (RC) of teeth submitted to endodontic retreatment due to prosthetic reasons and without evidence of apical periodontitis.

Material and methods

Twenty teeth referred to endodontic retreatment due to prosthetic reasons and without evidence of apical periodontitis were included in this study. Gutta-percha (GP) was collected from each third of the RC. After GP removal, microbial samples were also collected from the full extension of RC using paper points and files. The samples were cultivated in Fastidious Anaerobe Agar and the colony-forming units (CFU/mL) were counted. They were also semi-quantitatively analyzed by checkerboard DNA–DNA hybridization using 40 DNA bacterial probes.

Results

Microorganisms were found in all samples. The coronal third of GP was more contaminated than the apical third (p ≤ 0.05). There was prevalence of Enterococcus hirae and Enterococcus faecalis in all RC thirds and also in the samples collected from the full extension of RCs.

Conclusion

Microorganisms were found in all cases referred to endodontic retreatment due to prosthetic reasons and without evidence of apical periodontitis. Enterococcus was the genus most frequently detected.

Clinical significance

An endodontic retreatment should be considered before replacing a prosthesis.

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Acknowledgments

We are thankful to Maicon R. Z. Passini from the Piracicaba Dental School - UNICAMP and Izilvânia M. Q. Barreto from Guarulhos University for the technical support. The authors would like to thank Espaço da Escrita – Coordenadoria Geral da Universidade - UNICAMP - for the language service provided.

Funding

The work was financially supported by the São Paulo Research Foundation (FAPESP process no. 2015/23479-5), the Coordination for the Improvement of Higher Education Personnel (CAPES, financial code 001), and the Brazilian National Council for Scientific and Technological Development (CNPq, process no. 308162/2014-5).

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Authors and Affiliations

  1. Department of Restorative Dentistry, Endodontics Division, Piracicaba Dental School, State University of Campinas-UNICAMP, Piracicaba, SP, Brazil

    Eloa C. Bicego-Pereira, Marlos Barbosa-Ribeiro, Adriana de-Jesus-Soares, Alexandre A. Zaia, Caio C. R. Ferraz, José Flavio A. Almeida, Marina A. Marciano & Brenda P. F. A. Gomes

  2. Department of Periodontology, Dental Research Division, Guarulhos University, Guarulhos, São Paulo, Brazil

    Marlos Barbosa-Ribeiro & Magda Feres

Authors
  1. Eloa C. Bicego-Pereira
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  2. Marlos Barbosa-Ribeiro
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  3. Adriana de-Jesus-Soares
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  4. Alexandre A. Zaia
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  5. Caio C. R. Ferraz
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  6. José Flavio A. Almeida
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  7. Marina A. Marciano
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  8. Magda Feres
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  9. Brenda P. F. A. Gomes
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Correspondence to Brenda P. F. A. Gomes.

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This article contains studies with human participants or animals performed by any of the authors. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Bicego-Pereira, E.C., Barbosa-Ribeiro, M., de-Jesus-Soares, A. et al. Evaluation of the presence of microorganisms from root canal of teeth submitted to retreatment due to prosthetic reasons and without evidence of apical periodontitis. Clin Oral Invest 24, 3243–3254 (2020). https://doi.org/10.1007/s00784-020-03200-z

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  • DOI: https://doi.org/10.1007/s00784-020-03200-z

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