Isovalerate is one of the key intermediates during anaerobic digestion treating protein-containing waste/wastewater. Investigating the effect of different kinds of inhibitors on isovalerate-degrading microbial community is necessary to develop measures for improving the effectiveness of the treatment plants. In the present study, dynamic changes in the isovalerate-degrading microbial community in presence of inhibitors (ammonium, sulfide, mixed ammonium and sulfide, and chlortetracycline (CTC)) were investigated using high-throughput sequencing of 16S rRNA gene. Our observations showed that the isovalerate-degrading microbial community responded differently to different inhibitors and that the isovalerate degradation and gas production were strongly repressed by each inhibitor. We found that sulfide inhibited both isovalerate oxidation followed by methanogenesis, while ammonium, mixed ammonium and sulfide, and CTC mainly inhibited isovalerate oxidation. Genera classified into Proteobacteria and Chloroflexi were less sensitive to inhibitors. The two dominant genera, which are potential syntrophic isovalerate oxidizers, exhibited different responses to inhibitors that the unclassified_Peptococcaceae_3 was more sensitive to inhibitors than the unclassified_Syntrophaceae. Upon comparison to acetoclastic methanogen Methanosaeta, hydrogenotrophic methanogens Methanoculleus and Methanobacterium were less sensitive to inhibitors.

异戊酸酯是厌氧消化处理含蛋白质的废水/废水中的关键中间体之一。研究各种抑制剂对异戊酸降解微生物群落的影响对于开发提高处理植物效力的措施是必要的。在本研究中，使用16S rRNA基因的高通量测序研究了在存在抑制剂（铵，硫化物，混合铵和硫化物以及金霉素（CTC））的情况下，异戊酸降解微生物群落的动态变化。我们的观察结果表明，异戊酸降解微生物群落对不同抑制剂的反应不同，并且每种抑制剂都强烈抑制异戊酸降解和产气。我们发现，硫化物抑制异戊酸的氧化，然后抑制甲烷生成，而铵，铵盐和硫化铵混合，以及四氯化碳主要抑制异戊酸的氧化。属分为变形杆菌和Chloroflexi对抑制剂的敏感性较低。这两个主要属是潜在的同养异戊酸氧化剂，它们对抑制剂表现出不同的响应，即未分类的拟球菌科_3比未分类的同食菌科对抑制剂更敏感。与乙酰破伤性产甲烷菌甲烷菌相比，氢营养型产甲烷菌甲烷菌和甲烷菌对抑制剂的敏感性较低。