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Response of Isovalerate-Degrading Methanogenic Microbial Community to Inhibitors

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Abstract

Isovalerate is one of the key intermediates during anaerobic digestion treating protein-containing waste/wastewater. Investigating the effect of different kinds of inhibitors on isovalerate-degrading microbial community is necessary to develop measures for improving the effectiveness of the treatment plants. In the present study, dynamic changes in the isovalerate-degrading microbial community in presence of inhibitors (ammonium, sulfide, mixed ammonium and sulfide, and chlortetracycline (CTC)) were investigated using high-throughput sequencing of 16S rRNA gene. Our observations showed that the isovalerate-degrading microbial community responded differently to different inhibitors and that the isovalerate degradation and gas production were strongly repressed by each inhibitor. We found that sulfide inhibited both isovalerate oxidation followed by methanogenesis, while ammonium, mixed ammonium and sulfide, and CTC mainly inhibited isovalerate oxidation. Genera classified into Proteobacteria and Chloroflexi were less sensitive to inhibitors. The two dominant genera, which are potential syntrophic isovalerate oxidizers, exhibited different responses to inhibitors that the unclassified_Peptococcaceae_3 was more sensitive to inhibitors than the unclassified_Syntrophaceae. Upon comparison to acetoclastic methanogen Methanosaeta, hydrogenotrophic methanogens Methanoculleus and Methanobacterium were less sensitive to inhibitors.

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Acknowledgments

This work was supported by the Ministry of Science and Technology of China (No. 2016YFE0127700) and the National Natural Science Foundation of China (No. 51678378).

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Authors and Affiliations

  1. College of Architecture and Environment, Sichuan University, No. 24, South Section 1, First Ring Road, Chengdu, 610065, Sichuan, China

    Jie Li, Hui-Zhong Wang, Yue Yi, Min Gou, Ya-Ting Chen & Yue-Qin Tang

  2. Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, Higashi 1-1-1, Tsukuba, Ibaraki, 305-8566, Japan

    Masaru Konishi Nobu

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  1. Jie Li
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Li, J., Wang, HZ., Yi, Y. et al. Response of Isovalerate-Degrading Methanogenic Microbial Community to Inhibitors. Appl Biochem Biotechnol 191, 1010–1026 (2020). https://doi.org/10.1007/s12010-020-03234-9

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