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Components of partial resistance to Plasmopara viticola enable complete phenotypic characterization of grapevine varieties.
Scientific Reports ( IF 3.8 ) Pub Date : 2020-01-17 , DOI: 10.1038/s41598-020-57482-0
Federica Bove 1 , Vittorio Rossi 1
Affiliation  

Six components of partial resistance (RCs) were studied in 15 grapevine varieties with partial resistance to Plasmopara viticola: (i) infection frequency (IFR, proportion of inoculation sites showing sporulation), (ii) latent period (LP50, degree-days between inoculation and appearance of 50% of the final number of sporulating lesions), (iii) lesion size (LS, area of single lesions in mm2), (iv) production of sporangia (SPOR, number of sporangia produced per lesion, and SPOR', number of sporangia produced per mm2 of lesion), (v) infectious period (IP, number of sporulation events on a lesion), and (vi) infectivity of sporangia (INF, infection efficiency of sporangia produced on resistant varieties). Artificial inoculation monocycle experiments were conducted for a 3-year period on leaves collected at leaf development, flowering, and fruit development. Compared to the susceptible variety 'Merlot', the partially resistant varieties showed reduced IFR, longer LP, smaller LS, fewer SPOR and SPOR', shorter IP, and lower INF. At leaf development, IFR, SPOR, and INF were higher and LP was shorter than at flowering and fruit development. RCs analysis through monocyclic experiments provides reliable assessments of the resistance response of grapevine accessions. The workload required for routine assessment in breeding programs could be reduced by measuring IFR and SPOR, while producing robust results.

中文翻译:

对葡萄纤维单胞菌具有部分抗性的成分能够对葡萄品种进行完整的表型鉴定。

在15个对葡萄小单胞菌有部分抵抗力的葡萄品种中研究了六种部分抵抗力(RCs):(i)感染频率(IFR,接种部位显示出孢子的比例),(ii)潜伏期(LP50,接种之间的天数)占最终形成孢子病变数量的50%),(iii)病变大小(LS,单个病变面积,以mm2为单位),(iv)孢子囊的产生(SPOR,每个病变产生的孢子囊数量,以及SPOR',每mm2病灶产生的孢子囊数量)(v)感染期(IP,病灶上的孢子形成事件数),以及(vi)孢​​子囊的感染性(INF,抗性品种产生的孢子囊的感染效率)在叶发育,开花,和水果发展。与易感品种“梅洛”相比,部分抗病品种的IFR降低,LP更长,LS更低,SPOR和SPOR'更少,IP缩短,INF降低。与开花和果实发育相比,叶片发育时的IFR,SPOR和INF较高,而LP较短。通过单环实验进行的RCs分析提供了对葡萄品种抗性响应的可靠评估。可以通过测量IFR和SPOR来减少育种计划中常规评估所需的工作量,同时产生可靠的结果。和INF较高,LP较开花和果实发育时短。通过单环实验进行的RCs分析提供了对葡萄品种抗性响应的可靠评估。可以通过测量IFR和SPOR来减少育种计划中常规评估所需的工作量,同时产生可靠的结果。和INF较高,LP较开花和果实发育时短。通过单环实验进行的RCs分析提供了对葡萄品种抗性反应的可靠评估。可以通过测量IFR和SPOR来减少育种计划中常规评估所需的工作量,同时产生可靠的结果。
更新日期:2020-01-17
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