The cytoplasmic male sterility (CMS) system is a useful tool for commercial hybrid cotton seed production. Two main CMS systems, CMS-D8 and CMS-D2, have been recognized with Rf2 and Rf1 as the restorer genes, respectively. The development of molecular markers tightly linked with restorer genes can facilitate the breeding of restorer lines. In this study, the InDel-1892 marker was developed to distinguish Rf2 and Rf1 simultaneously. Sequence alignment implied that CMS-D8-Rf2 has a 32 bp insertion and that CMS-D2-Rf1 has a 186 bp insertion at the InDel-1892 locus. The codominant marker was co-segregated with Rf1 and Rf2. Hence, this marker can be used for tracing Rf1 and Rf2 simultaneously and identifying the allele status at the restorer gene locus. The results of this study will facilitate efficient marker-assisted selection for restorer lines and hybrids of CMS systems.

中文翻译：

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与棉花育性恢复基因CMS-D8和CMS-D2相关的InDel标记的开发和利用。

细胞质雄性不育（CMS）系统是用于商业杂交棉种子生产的有用工具。已经认识到两个主要的CMS系统CMS-D8和CMS-D2，分别以Rf2和Rf1作为恢复基因。与恢复基因紧密联系的分子标记的发展可以促进恢复系的育种。在这项研究中，开发了InDel-1892标记以同时区分Rf2和Rf1。序列比对暗示在InDel-1892基因座上CMS-D8-Rf2具有32bp的插入，而CMS-D2-Rf1具有186bp的插入。共同标记与Rf1和Rf2共分离。因此，该标记可用于同时追踪Rf1和Rf2，并在恢复基因位点鉴定等位基因状态。