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Development and utilization of an InDel marker linked to the fertility restorer genes of CMS-D8 and CMS-D2 in cotton

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Abstract

The cytoplasmic male sterility (CMS) system is a useful tool for commercial hybrid cotton seed production. Two main CMS systems, CMS-D8 and CMS-D2, have been recognized with Rf2 and Rf1 as the restorer genes, respectively. The development of molecular markers tightly linked with restorer genes can facilitate the breeding of restorer lines. In this study, the InDel-1892 marker was developed to distinguish Rf2 and Rf1 simultaneously. Sequence alignment implied that CMS-D8-Rf2 has a 32 bp insertion and that CMS-D2-Rf1 has a 186 bp insertion at the InDel-1892 locus. The codominant marker was co-segregated with Rf1 and Rf2. Hence, this marker can be used for tracing Rf1 and Rf2 simultaneously and identifying the allele status at the restorer gene locus. The results of this study will facilitate efficient marker-assisted selection for restorer lines and hybrids of CMS systems.

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Funding

The research was supported by grants from the National Natural Science Foundation of China (31871679) and the Central Public-interest Scientific Institution Basal Research Fund (1610162019010303).

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  1. Juanjuan Feng and Haiyong Zhu have contributed equally to this work.

Authors and Affiliations

  1. Zhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou, 450000, Henan, China

    Juanjuan Feng & Jianyong Wu

  2. State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, 455000, Henan, China

    Juanjuan Feng, Haiyong Zhu, Meng Zhang, Xuexian Zhang, Liping Guo, Tingxiang Qi, Huini Tang, Hailin Wang, Xiuqin Qiao, Bingbing Zhang, Kashif Shahzad, Chaozhu Xing & Jianyong Wu

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  1. Juanjuan Feng
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Fig. S1 Polymorphic analysis of the InDel-7138 marker in the CMS-D8 and the CMS-D2 systems. Supplementary Material 1 (PDF 346 kb)

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Feng, J., Zhu, H., Zhang, M. et al. Development and utilization of an InDel marker linked to the fertility restorer genes of CMS-D8 and CMS-D2 in cotton. Mol Biol Rep 47, 1275–1282 (2020). https://doi.org/10.1007/s11033-019-05240-5

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