Abstract

Glioma is the most aggressive primary brain tumor. We have previously provided evidence that IFITM3 promoted glioma cells migration. However, the mechanism of how IFITM3 regulates glioma cells invasion and whether IFITM3 participates in TGF-β-mediated glioma invasion are still unknown. In this paper, we proved that IFITM3 was notably up-regulated in glioma tissues. Knockdown of IFITM3 suppressed STAT3 phosphorylation in vitro, and a specific STAT3 inhibitor AG490 reversed IFITM3-induced invasion of glioma cells. Furthermore, IFITM3 expression was induced by TGF-β in glioma and IFITM3 knockdown abolished TGF-β-mediated glioma cells invasion. Collectively, the results indicate that IFITM3/STAT3 axis may promote TGF-β-induced glioma cells invasion. This study provided some suggestions for the clinical treatment of the brain tumor.

中文翻译：

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IFITM3 / STAT3轴促进神经胶质瘤细胞侵袭并受TGF-β调节

摘要

胶质瘤是最具侵略性的原发性脑肿瘤。我们先前已经提供了IFITM3促进神经胶质瘤细胞迁移的证据。然而，IFITM3如何调节神经胶质瘤细胞侵袭的机制以及IFITM3是否参与TGF-β介导的神经胶质瘤侵袭的机制仍然未知。在本文中，我们证明了IFITM3在神经胶质瘤组织中明显上调。击倒IFITM3抑制体外STAT3磷酸化，和特定的STAT3抑制剂AG490逆转了IFITM3诱导的胶质瘤细胞侵袭。此外，TGF-β在神经胶质瘤中诱导了IFITM3的表达，而IFITM3的敲除消除了TGF-β介导的神经胶质瘤细胞的侵袭。总体而言，该结果表明IFITM3 / STAT3轴可能促进TGF-β诱导的神经胶质瘤细胞侵袭。该研究为脑肿瘤的临床治疗提供了一些建议。