Campylobacter jejuni is the most common bacterial cause of foodborne diarrheal disease worldwide and is among the antimicrobial resistant “priority pathogens” that pose greatest threat to public health. The genomes of two C. jejuni isolated from poultry meat sold on the retail market in Southern Brazil phenotypically characterized as multidrug-resistant (CJ100) and susceptible (CJ104) were sequenced and analyzed by bioinformatic tools. The isolates CJ100 and CJ104 showed distinct multilocus sequence types (MLST). Comparative genomic analysis revealed a large number of single nucleotide polymorphisms, rearrangements, and inversions in both genomes, in addition to virulence factors, genomic islands, prophage sequences, and insertion sequences. A circular 103-kilobase megaplasmid carrying virulence factors was identified in the genome of CJ100, in addition to resistance mechanisms to aminoglycosides, beta-lactams, macrolides, quinolones, and tetracyclines. The molecular characterization of distinct phenotypes of foodborne C. jejuni and the discovery of a novel virulence megaplasmid provide useful data for pan-genome and large-scale studies to monitor the virulent C. jejuni in poultry meat is warranted.

中文翻译：

---

从巴西家禽肉中分离出的空肠弯曲菌：计算机分析和两种具有不同抗生素敏感性表型的菌株的基因组特征

空肠弯曲杆菌是全世界食源性腹泻病的最常见细菌原因，并且是对公众健康构成最大威胁的抗药性“优先病原体”。两个空肠弯曲菌的基因组从表型上被表征为耐多药（CJ100）和易感（CJ104）的巴西南部零售市场出售的禽肉中分离得到的白蛋白进行了测序，并通过生物信息学工具进行了分析。分离株CJ100和CJ104显示出不同的多基因座序列类型（MLST）。比较基因组分析显示，除了毒力因子，基因组岛，噬菌体序列和插入序列外，两个基因组中还存在大量的单核苷酸多态性，重排和倒位。除了对氨基糖苷类，β-内酰胺类，大环内酯类，喹诺酮类和四环素类的耐药机制外，在CJ100的基因组中还鉴定出了带有毒力因子的环状103千碱基的大质粒。食源性空肠弯曲菌不同表型的分子表征新型毒力大质粒的发现为全基因组研究和监视禽肉中空肠弯曲杆菌的大规模研究提供了有用的数据。