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Campylobacter jejuni isolated from poultry meat in Brazil: in silico analysis and genomic features of two strains with different phenotypes of antimicrobial susceptibility

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Abstract

Campylobacter jejuni is the most common bacterial cause of foodborne diarrheal disease worldwide and is among the antimicrobial resistant “priority pathogens” that pose greatest threat to public health. The genomes of two C. jejuni isolated from poultry meat sold on the retail market in Southern Brazil phenotypically characterized as multidrug-resistant (CJ100) and susceptible (CJ104) were sequenced and analyzed by bioinformatic tools. The isolates CJ100 and CJ104 showed distinct multilocus sequence types (MLST). Comparative genomic analysis revealed a large number of single nucleotide polymorphisms, rearrangements, and inversions in both genomes, in addition to virulence factors, genomic islands, prophage sequences, and insertion sequences. A circular 103-kilobase megaplasmid carrying virulence factors was identified in the genome of CJ100, in addition to resistance mechanisms to aminoglycosides, beta-lactams, macrolides, quinolones, and tetracyclines. The molecular characterization of distinct phenotypes of foodborne C. jejuni and the discovery of a novel virulence megaplasmid provide useful data for pan-genome and large-scale studies to monitor the virulent C. jejuni in poultry meat is warranted.

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Acknowledgements

This study was financed in party by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES—Finance Code 001), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq-Grant 309101/2016-6), and Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS-Grant 17/2551-0000956-8).

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Authors and Affiliations

  1. Centro de Desenvolvimento Tecnológico, Núcleo de Biotecnologia, Universidade Federal de Pelotas, Pelotas, RS, Brazil

    Simone de Fátima Rauber Würfel, Sérgio Jorge, Natasha Rodrigues de Oliveira, Frederico Schmitt Kremer, Christian Domingues Sanchez, Vinícius Farias Campos, Luciano da Silva Pinto & Odir Antônio Dellagostin

  2. Departamento de Ciência e Tecnologia Agroindustrial, Faculdade de Agronomia Eliseu Maciel, Universidade Federal de Pelotas, Pelotas, RS, Brazil

    Wladimir Padilha da Silva

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  1. Simone de Fátima Rauber Würfel
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  2. Sérgio Jorge
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  9. Odir Antônio Dellagostin
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Correspondence to Sérgio Jorge.

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Nucleotide sequence accession numbers

Sequence data from this article have been deposited with the DDBJ/EMBL/GenBank Data Libraries under Accessions CP023343.1 (chromosome, C. jejuni strain 104), CP023446.1 (chromosome, C. jejuni strain 100), and CP023447.1 (plasmid pJc100).

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de Fátima Rauber Würfel, S., Jorge, S., de Oliveira, N.R. et al. Campylobacter jejuni isolated from poultry meat in Brazil: in silico analysis and genomic features of two strains with different phenotypes of antimicrobial susceptibility. Mol Biol Rep 47, 671–681 (2020). https://doi.org/10.1007/s11033-019-05174-y

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