The objective of this study was to track intentionally inoculated Leuconostoc mesenteroides (11251) and Lactobacillus brevis (B151) strains in kimchi using random amplified polymorphic DNA (RAPD), repetitive element palindromic PCR (rep-PCR), and comparative housekeeping gene sequences analysis. The 16S rRNA gene provided species-level information for 30 colonies randomly picked from kimchi inoculated with strains 11251 and B151. Out of 30 colonies, one colony was matched to strain 11251, and two colonies were found identical to strain B151 reference strain in inoculated kimchi. Notably, among the three tools, strain 11251 was best tracked by comparative gene sequence analysis, while strain B151 tracked by all three tools. Our results suggest that the gene sequence analysis is a more reliable tool for tracking of desired strains than RAPD and rep-PCR. Based on the findings, it is recommended that gene sequence analysis could be used to avoid misuse of industrially useful strains within the growing food industry.

这项研究的目的是追踪故意接种的肠膜明串珠菌（11251）和短乳杆菌（B151）使用随机扩增多态性DNA（RAPD），重复元素回文PCR（rep-PCR）和比较看家基因序列分析对泡菜中的菌株进行检测。16S rRNA基因提供了从菌株11251和B151接种的泡菜中随机挑选的30个菌落的物种水平信息。在30个菌落中，有一个菌落与11251菌株匹配，发现两个菌落与接种的泡菜中的B151参考菌株相同。值得注意的是，在这三个工具中，通过比较基因序列分析可以最好地跟踪菌株11251，而通过这三个工具可以跟踪菌株B151。我们的结果表明，与RAPD和rep-PCR相比，基因序列分析是追踪所需菌株的更可靠工具。根据调查结果，